Evidence map›Paper›PMID 25379593›Full record

ArticleJournal of medical primatology2014

Development and optimization of a non-enzymatic method of leukocyte isolation from macaque tissues.

Lara E Pereira, Natalia Makarova, Charles Dobard, Rachael D Aubert, Priya Srinivasan, Janet McNicholl, James M Smith

Open access · greenAbstract read
In one paragraph

Article in Journal of medical primatology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 85% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Lara E Pereira
Natalia Makarova
Charles Dobard
Rachael D Aubert
Priya Srinivasan
Janet McNicholl
James M Smith
Centers for Disease Control and Prevention · US

Funding

Intramural CDC HHS CC999999
6 · The paper itself

Abstract

BACKGROUND AND

methodsCell isolation from macaque tissues involves laborious enzymatic digestion. The Medimachine provides a simpler, quicker non-enzymatic method, yielding 1.5–5 million cells/g of vaginal or rectal tissue from pigtailed macaques. RESULTS AND

conclusionsFlow cytometry analysis of the two methods revealed similar levels of cell viability and most major cell lineage and activation markers.

Indexed as

AnimalsCell SeparationCell SurvivalFemaleFlow CytometryLeukocytesMacaca nemestrinaRectumVagina

Identifiers

PMID25379593
PMCPMC6711605
OpenAlexW2158526231

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.