ArticleResults in immunology2014
Establishment of c-myc-immortalized Kupffer cell line from a C57BL/6 mouse strain.
Article in Results in immunology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Characterization of novel macrophage cell lines derived from goat and sheep blood and their susceptibility to ruminant virus infections.Microbiology spectrum · 2026Article
- Effect of antibiotics on Kupffer cell immunometabolism relative to intracellular killing ofmBio · 2025Article
- Therapeutic effects of adipose tissue-derived mesenchymal stem cells on ER stress in a murine model of metabolic dysfunction-associated steatohepatitis: an in vivo and in vitro study.Stem cell research & therapy · 2025Article
- Comparative in vitro efficacy of antibiotics against the intracellular reservoir of Staphylococcus aureus.The Journal of antimicrobial chemotherapy · 2024Article
- Opportunities and considerations for studying liver disease with microphysiological systems on a chip.Lab on a chip · 2023Review
- Generation and characterization of genome-modified chondrocyte-like cells from the zebra finch cell line immortalized by c-MYC expression.Frontiers in zoology · 2022Article
- Downregulation of miR-122-5p Activates Glycolysis via PKM2 in Kupffer Cells of Rat and Mouse Models of Non-Alcoholic Steatohepatitis.International journal of molecular sciences · 2022Article
- Distinguishing functional exosomes and other extracellular vesicles as a nucleic acid cargo by the anion-exchange method.Journal of extracellular vesicles · 2022Article
- Isolation and immortalization of macrophages derived from fetal porcine small intestine and their susceptibility to porcine viral pathogen infections.Frontiers in veterinary science · 2022Article
- LECT2 as a hepatokine links liver steatosis to inflammation via activating tissue macrophages in NASH.Scientific reports · 2021Article
- Dimethyl fumarate ameliorates hepatic inflammation in alcohol related liver disease.Liver international : official journal of the International Association for the Study of the Liver · 2020Article
- Creative use of analytical techniques and high-throughput technology to facilitate safety assessment of engineered nanomaterials.Analytical and bioanalytical chemistry · 2018Review
- The small molecule drug diminazene aceturate inhibits liver injury and biliary fibrosis in mice.Scientific reports · 2018Article
- Growth differentiation factor 15 ameliorates nonalcoholic steatohepatitis and related metabolic disorders in mice.Scientific reports · 2018Article
- Liver 'organ on a chip'.Experimental cell research · 2018Review
- Angiotensin II subtype 1a receptor signaling in resident hepatic macrophages induces liver metastasis formation.Cancer science · 2017Article
- Efficient and simple approach to in vitro culture of primary epithelial cancer cells.Bioscience reports · 2016Article
- Infusion of Kupffer Cells Expanded inCell transplantationArticle
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6 authors.
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No grant is acknowledged in the PubMed record.
Abstract
We recently demonstrated in several mammalian species, a novel procedure to obtain liver-macrophages (Kupffer cells) in sufficient numbers and purity using a mixed primary culture of hepatocytes. In this study, we applied this method to the C57BL/6 mouse liver and established an immortalized Kupffer cell line from this mouse strain. The hepatocytes from the C57BL/6 adult mouse liver were isolated by a two-step collagenase perfusion method and cultured in T25 culture flasks. Similar to our previous studies, the mouse hepatocytes progressively changed their morphology into a fibroblastic appearance after a few days of culture. After 7-10 days of culture, Kupffer-like cells, which were contaminants in the hepatocyte fraction at the start of the culture, actively proliferated on the mixed fibroblastic cell sheet. At this stage, a retroviral vector containing the human c-myc oncogene and neomycin resistance gene was introduced into the mixed culture. Gentle shaking of the culture flask, followed by the transfer and brief incubation of the culture supernatant, resulted in a quick and selective adhesion of Kupffer cells to a plastic dish surface. After selection with G418 and cloning by limiting dilutions, a clonal cell line (KUP5) was established. KUP5 cells displayed typical macrophage morphology and were stably passaged at 4-5 days intervals for more than 5 months, with a population doubling time of 19 h. KUP5 cells are immunocytochemically positive for mouse macrophage markers, such as Mac-1, F4/80. KUP5 cells exhibited substantial phagocytosis of polystyrene microbeads and the release of inflammatory cytokines upon lipopolysaccharide stimulation. Taken together, KUP5 cells provide a useful means to study the function of Kupffer cells in vitro.
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