ReviewComputational and structural biotechnology journal2014
A review of metabolic and enzymatic engineering strategies for designing and optimizing performance of microbial cell factories.
Review in Computational and structural biotechnology journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed.
- Biosynthesis and Microbial Production of Carminic Acid: From Pathway Elucidation to Synthetic Biology.Microorganisms · 2026Review
- Stress-induced remodeling of yeast amino acid pools occurs within conserved physicochemical constraints.Folia microbiologica · 2026Article
- A highly active Burkholderia polyketoacyl-CoA thiolase for production of triacetic acid lactone.Nature communications · 2025Article
- Green Solutions for Food Safety: The Emerging Applications of Zearalenone-Degrading Enzymes.Foods (Basel, Switzerland) · 2025Review
- Chemoenzymatic synthesis.Communications chemistry · 2025Article
- BayesianSSA: a Bayesian statistical model based on structural sensitivity analysis for predicting responses to enzyme perturbations in metabolic networks.BMC bioinformatics · 2024Article
- Engineering of a Substrate Affinity Reduced S-Adenosyl-methionine Synthetase as a Novel Biosensor for Growth-Coupling Selection of L-Methionine Overproducers.Applied biochemistry and biotechnology · 2024Article
- Integrated transcriptomics and metabolomics provide insights into the biosynthesis of militarine in the cell suspension culture system of Bletilla striata.Advanced biotechnology · 2024Article
- Exploring Emergent Properties in Enzymatic Reaction Networks: Design and Control of Dynamic Functional Systems.Chemical reviews · 2024Review
- FastKnock: an efficient next-generation approach to identify all knockout strategies for strain optimization.Microbial cell factories · 2024Article
- Advancing microbiota therapeutics: the role of synthetic biology in engineering microbial communities for precision medicine.Frontiers in bioengineering and biotechnology · 2024Review
- Rational design of GDP‑D‑mannose mannosyl hydrolase for microbial L‑fucose production.Microbial cell factories · 2023Article
- Transporter Engineering in Microbial Cell Factory Boosts Biomanufacturing Capacity.Biodesign research · 2022Review
- Systems biology approaches integrated with artificial intelligence for optimized metabolic engineering.Metabolic engineering communications · 2020Review
- Application of combinatorial optimization strategies in synthetic biology.Nature communications · 2020Review
- Development of a metabolic pathway transfer and genomic integration system for the syngas-fermenting bacteriumBiotechnology for biofuels · 2019Article
- Systems metabolic engineering ofJournal of biological engineering · 2019Article
- Application of the thermostable β-galactosidase, BgaB, from Geobacillus stearothermophilus as a versatile reporter under anaerobic and aerobic conditions.AMB Express · 2017Article
- Whole cell biocatalysts: essential workers from Nature to the industry.Microbial biotechnology · 2017Review
- Improvement Strategies, Cost Effective Production, and Potential Applications of Fungal Glucose Oxidase (GOD): Current Updates.Frontiers in microbiology · 2017Review
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microbial cell factories (MCFs) are of considerable interest to convert low value renewable substrates to biofuels and high value chemicals. This review highlights the progress of computational models for the rational design of an MCF to produce a target bio-commodity. In particular, the rational design of an MCF involves: (i) product selection, (ii) de novo biosynthetic pathway identification (i.e., rational, heterologous, or artificial), (iii) MCF chassis selection, (iv) enzyme engineering of promiscuity to enable the formation of new products, and (v) metabolic engineering to ensure optimal use of the pathway by the MCF host. Computational tools such as (i) de novo biosynthetic pathway builders, (ii) docking, (iii) molecular dynamics (MD) and steered MD (SMD), and (iv) genome-scale metabolic flux modeling all play critical roles in the rational design of an MCF. Genome-scale metabolic flux models are of considerable use to the design process since they can reveal metabolic capabilities of MCF hosts. These can be used for host selection as well as optimizing precursors and cofactors of artificial de novo biosynthetic pathways. In addition, recent advances in genome-scale modeling have enabled the derivation of metabolic engineering strategies, which can be implemented using the genomic tools reviewed here as well.
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Registered trials
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