ArticleBiomicrofluidics2014
Examination of the role of transient receptor potential vanilloid type 4 in endothelial responses to shear forces.
Article in Biomicrofluidics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 35 citations in OpenAlex.
- Understanding Fascial Tissue on the Molecular Level-How Its Unique Properties Enable Adaptation or Dysfunction.International journal of molecular sciences · 2025Review
- A Highly Adaptable Hydrogen Bond Re-Orientation (HyBRO) Strategy for Multiscale Vasculature Fabrication.Advanced materials (Deerfield Beach, Fla.) · 2025Article
- From Mechanoelectric Conversion to Tissue Regeneration: Translational Progress in Piezoelectric Materials.Advanced materials (Deerfield Beach, Fla.) · 2025Review
- Force versus Response: Methods for Activating and Characterizing Mechanosensitive Ion Channels and GPCRs.Advanced healthcare materials · 2024Review
- A Review of Functional Analysis of Endothelial Cells in Flow Chambers.Journal of functional biomaterials · 2022Review
- Remote and Selective Control of Astrocytes by Magnetomechanical Stimulation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Microfluidic models of the human circulatory system: versatile platforms for exploring mechanobiology and disease modeling.Biophysical reviews · 2021Review
- Computational and experimental studies of a cell-imprinted-based integrated microfluidic device for biomedical applications.Scientific reports · 2021Article
- Asynchronous generation of oil droplets using a microfluidic flow focusing system.Scientific reports · 2019Article
- The TRPV4 Agonist GSK1016790A Regulates the Membrane Expression of TRPV4 Channels.Frontiers in pharmacology · 2019Article
- "Do-it-in-classroom" fabrication of microfluidic systems by replica moulding of pasta structures.Biomicrofluidics · 2018Article
- Shear Stress Regulates TRPV4 Channel Clustering and Translocation from Adherens Junctions to the Basal Membrane.Scientific reports · 2017Article
- Article
- Concurrent shear stress and chemical stimulation of mechano-sensitive cells by discontinuous dielectrophoresis.Biomicrofluidics · 2016Article
- Shear stress mediates exocytosis of functional TRPV4 channels in endothelial cells.Cellular and molecular life sciences : CMLS · 2016Article
- Analysing calcium signalling of cells under high shear flows using discontinuous dielectrophoresis.Scientific reports · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Shear stress is the major mechanical force applied on vascular endothelial cells by blood flow, and is a crucial factor in normal vascular physiology and in the development of some vascular pathologies. The exact mechanisms of cellular mechano-transduction in mammalian cells and tissues have not yet been elucidated, but it is known that mechanically sensitive receptors and ion channels play a crucial role. This paper describes the use of a novel and efficient microfluidic device to study mechanically-sensitive receptors and ion channels in vitro, which has three independent channels from which recordings can be made and has a small surface area such that fewer cells are required than for conventional flow chambers. The contoured channels of the device enabled examination of a range of shear stresses in one field of view, which is not possible with parallel plate flow chambers and other previously used devices, where one level of flow-induced shear stress is produced per fixed flow-rate. We exposed bovine aortic endothelial cells to different levels of shear stress, and measured the resulting change in intracellular calcium levels ([Ca(2+)]i) using the fluorescent calcium sensitive dye Fluo-4AM. Shear stress caused an elevation of [Ca(2+)]i that was proportional to the level of shear experienced. The response was temperature dependant such that at lower temperatures more shear stress was required to elicit a given level of calcium signal and the magnitude of influx was reduced. We demonstrated that shear stress-induced elevations in [Ca(2+)]i are largely due to calcium influx through the transient receptor potential vanilloid type 4 ion channel.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.