ArticleBiomicrofluidics2014
Characterization of microfluidic shear-dependent epithelial cell adhesion molecule immunocapture and enrichment of pancreatic cancer cells from blood cells with dielectrophoresis.
Article in Biomicrofluidics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The trial behind it
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Who cites it
15 citing papers in PubMed, 25 citations in OpenAlex.
- Recent advances in integrated biophysical and biochemical microfluidic methods for circulating tumor cells isolation and analysis.Fundamental research · 2026Review
- A Viscosity Model for Blood Flows in Narrow Channels at High Reynolds Numbers Incorporating a Cell-Free Layer.Micromachines · 2026Article
- Dielectrophoretic enrichment of live chemo-resistant circulating-like pancreatic cancer cells from media of drug-treated adherent cultures of solid tumors.Lab on a chip · 2024Article
- Blood Particle Separation Using Dielectrophoresis in A Novel Microchannel: A Numerical Study.Cell journal · 2020Article
- Liquid biopsy and minimal residual disease - latest advances and implications for cure.Nature reviews. Clinical oncology · 2019Review
- Enrichment and single-cell analysis of circulating tumor cells.Chemical science · 2017Article
- Automated electrorotation shows electrokinetic separation of pancreatic cancer cells is robust to acquired chemotherapy resistance, serum starvation, and EMT.Biomicrofluidics · 2016Article
- Emerging microfluidic devices for cancer cells/biomarkers manipulation and detection.IET nanobiotechnology · 2016Review
- Concurrent shear stress and chemical stimulation of mechano-sensitive cells by discontinuous dielectrophoresis.Biomicrofluidics · 2016Article
- Article
- Microfluidic Sample Preparation for Single Cell Analysis.Analytical chemistry · 2016Review
- Microfluidic platform for separation and extraction of plasma from whole blood using dielectrophoresis.Biomicrofluidics · 2015Article
- A hybrid dielectrophoretic system for trapping of microorganisms from water.Biomicrofluidics · 2015Article
- Enhancing sensitivity and specificity in rare cell capture microdevices with dielectrophoresis.Biomicrofluidics · 2015Article
- Three dimensional passivated-electrode insulator-based dielectrophoresis.Biomicrofluidics · 2015Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Current microfluidic techniques for isolating circulating tumor cells (CTCs) from cancer patient blood are limited by low capture purity, and dielectrophoresis (DEP) has the potential to complement existing immunocapture techniques to improve capture performance. We present a hybrid DEP and immunocapture Hele-Shaw flow cell to characterize DEP's effects on immunocapture of pancreatic cancer cells (Capan-1, PANC-1, and BxPC-3) and peripheral blood mononuclear cells (PBMCs) with an anti-EpCAM (epithelial cell adhesion molecule) antibody. By carefully specifying the applied electric field frequency, we demonstrate that pancreatic cancer cells are attracted to immunocapture surfaces by positive DEP whereas PBMCs are repelled by negative DEP. Using an exponential capture model to interpret our capture data, we show that immunocapture performance is dependent on the applied DEP force sign and magnitude, cell surface EpCAM expression level, and shear stress experienced by cells flowing in the capture device. Our work suggests that DEP can not only repel contaminating blood cells but also enhance capture of cancer cell populations that are less likely to be captured by traditional immunocapture methods. This combination of DEP and immunocapture techniques to potentially increase CTC capture purity can facilitate subsequent biological analyses of captured CTCs and research on cancer metastasis and drug therapies.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.