ArticleBiotechnology for biofuels2014
The exometabolome of Clostridium thermocellum reveals overflow metabolism at high cellulose loading.
Article in Biotechnology for biofuels, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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Who cites it
58 citing papers in PubMed.
- Effects of heterologous expression of glycolysis enzymes on product formation inMetabolic engineering communications · 2026Article
- EngineeringMetabolic engineering communications · 2026Article
- Metabolic imbalance limits fermentation in microbes engineered for high-titer ethanol production.mSystems · 2026Article
- EngineeringbioRxiv : the preprint server for biology · 2025Article
- Rhamnogalacturonan I is a recalcitrant pectin domain during Clostridium thermocellum-mediated deconstruction of switchgrass biomass.Biotechnology for biofuels and bioproducts · 2025Article
- Thermodynamics shapes themBio · 2025Article
- Thermophilic site-specific recombination system for rapid insertion of heterologous DNA into the Clostridium thermocellum chromosome.Journal of industrial microbiology & biotechnology · 2024Article
- Pyrophosphate-free glycolysis in Clostridium thermocellum increases both thermodynamic driving force and ethanol titers.Biotechnology for biofuels and bioproducts · 2024Article
- Expression and characterization of monofunctional alcohol dehydrogenase enzymes inMetabolic engineering communications · 2024Article
- The potential of native and engineered Clostridia for biomass biorefining.Frontiers in bioengineering and biotechnology · 2024Review
- Kinetic characterization of annotated glycolytic enzymes present in cellulose-fermenting Clostridium thermocellum suggests different metabolic roles.Biotechnology for biofuels and bioproducts · 2023Article
- Characterization and Amelioration of Filtration Difficulties Encountered in Metabolomic Studies of Clostridium thermocellum at Elevated Sugar Concentrations.Applied and environmental microbiology · 2023Article
- The Roles of Nicotinamide Adenine Dinucleotide Phosphate Reoxidation and Ammonium Assimilation in the Secretion of Amino Acids as Byproducts of Clostridium thermocellum.Applied and environmental microbiology · 2023Article
- Ethanol tolerance of Clostridium thermocellum: the role of chaotropicity, temperature and pathway thermodynamics on growth and fermentative capacity.Microbial cell factories · 2022Article
- Increasing the Thermodynamic Driving Force of the Phosphofructokinase Reaction inApplied and environmental microbiology · 2022Article
- Metaproteomics reveals enzymatic strategies deployed by anaerobic microbiomes to maintain lignocellulose deconstruction at high solids.Nature communications · 2022Article
- Multiplex knockout of trichome-regulating MYB duplicates in hybrid poplar using a single gRNA.Plant physiology · 2022Article
- Plant biomass fermentation by the extreme thermophile Caldicellulosiruptor bescii for co-production of green hydrogen and acetone: Technoeconomic analysis.Bioresource technology · 2022Article
- Composition and yield of non-cellulosic and cellulosic sugars in soluble and particulate fractions during consolidated bioprocessing of poplar biomass by Clostridium thermocellum.Biotechnology for biofuels and bioproducts · 2022Article
- Declining carbohydrate solubilization with increasing solids loading during fermentation of cellulosic feedstocks by Clostridium thermocellum: documentation and diagnostic tests.Biotechnology for biofuels and bioproducts · 2022Article
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8 authors.
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Abstract
backgroundClostridium thermocellum is a model thermophilic organism for the production of biofuels from lignocellulosic substrates. The majority of publications studying the physiology of this organism use substrate concentrations of ≤10 g/L. However, industrially relevant concentrations of substrate start at 100 g/L carbohydrate, which corresponds to approximately 150 g/L solids. To gain insight into the physiology of fermentation of high substrate concentrations, we studied the growth on, and utilization of high concentrations of crystalline cellulose varying from 50 to 100 g/L by C. thermocellum.
resultsUsing a defined medium, batch cultures of C. thermocellum achieved 93% conversion of cellulose (Avicel) initially present at 100 g/L. The maximum rate of substrate utilization increased with increasing substrate loading. During fermentation of 100 g/L cellulose, growth ceased when about half of the substrate had been solubilized. However, fermentation continued in an uncoupled mode until substrate utilization was almost complete. In addition to commonly reported fermentation products, amino acids - predominantly L-valine and L-alanine - were secreted at concentrations up to 7.5 g/L. Uncoupled metabolism was also accompanied by products not documented previously for C. thermocellum, including isobutanol, meso- and RR/SS-2,3-butanediol and trace amounts of 3-methyl-1-butanol, 2-methyl-1-butanol and 1-propanol. We hypothesize that C. thermocellum uses overflow metabolism to balance its metabolism around the pyruvate node in glycolysis.
conclusionsC. thermocellum is able to utilize industrially relevant concentrations of cellulose, up to 93 g/L. We report here one of the highest degrees of crystalline cellulose utilization observed thus far for a pure culture of C. thermocellum, the highest maximum substrate utilization rate and the highest amount of isobutanol produced by a wild-type organism.
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