ArticleJournal of toxicologic pathology2014
Intratracheal instillation methods and the distribution of administered material in the lung of the rat.
Article in Journal of toxicologic pathology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Multi-element Distribution Profile in Rats: Effects of Inhalable Intratracheal Instillation of Cr(VI).Biological trace element research · 2026Article
- Recent strategies for enhanced delivery of mRNA to the lungs.Nanomedicine (London, England) · 2025Review
- Comparison of Single and Multiple Intratracheal Administrations for Pulmonary Toxic Responses of Multi-Walled Carbon Nanotubes in Rats.Nanomaterials (Basel, Switzerland) · 2024Article
- Lung-Specific mRNA Delivery Enabled by Sulfonium Lipid Nanoparticles.Nano letters · 2024Article
- The use of LA-ICP-MS as an auxiliary tool to assess the pulmonary toxicity of molybdenum(IV) sulfide (MoSInternational journal of occupational medicine and environmental health · 2024Article
- Lung versus gut exposure to air pollution particles differentially affect metabolic health in mice.Particle and fibre toxicology · 2023Article
- Route of administration significantly affects particle deposition and cellular recruitment.PloS one · 2023Article
- Recrystallization of Adenosine for Localized Drug Delivery.Molecular pharmaceutics · 2022Article
- Exon-skipping antisense oligonucleotides for cystic fibrosis therapy.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Comparison of direct and indirect models of early induced acute lung injury.Intensive care medicine experimental · 2020Article
- Video laryngoscopic oral intubation in rats: a simple and effective method.American journal of physiology. Lung cellular and molecular physiology · 2020Article
- Method for Pulmonary Administration Using Negative Pressure Generated by Inspiration in Mice.Pharmaceutics · 2020Article
- Inter-laboratory comparison of pulmonary lesions induced by intratracheal instillation of NiO nanoparticle in rats: Histopathological examination results.Journal of occupational health · 2020Article
- Transbronchial Invasion and Proliferation of Leptospira interrogans in Lung without Inflammatory Cell Infiltration in a Hamster Model.Infection and immunity · 2019Article
- Development of experimental silicosis in inbred and outbred mice depends on instillation volume.Scientific reports · 2019Article
- Inhalation Exposure Analysis of Lung-Inhalable Particles in an Approximate Rat Central Airway.International journal of environmental research and public health · 2019Article
- Basic study of intratracheal instillation study of nanomaterials for the estimation of the hazards of nanomaterials.Industrial health · 2018Article
- Dual-Isotope SPECT/CT Imaging of the Tuberculosis Subunit Vaccine H56/CAF01: Induction of Strong Systemic and Mucosal IgA and T-Cell Responses in Mice Upon Subcutaneous Prime and Intrapulmonary Boost Immunization.Frontiers in immunology · 2018Article
- Systematic Assessment of Strategies for Lung-targeted Delivery of MicroRNA Mimics.Theranostics · 2018Article
- The Influence of Quercetin on Maternal Immunity, Oxidative Stress, and Inflammation in Mice with Exposure of Fine Particulate Matter during Gestation.International journal of environmental research and public health · 2017Article
Corrections and comments
- Erratum issued
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Intratracheal instillation is widely used for respiratory toxicity tests in experimental animals. However, there are wide variations in the techniques used for instillation, and it is thus difficult to compare the results obtained using different techniques. To examine the effect of instillation methods, we compared the distribution of a test substance in the lungs of rats after intratracheal instillations under various conditions. Rats received an intratracheal instillation of 0.3 mL of india ink suspension under different conditions as follows: 3 different angles of body restraint, 0° (supine horizontal), 45° (supine head up) and 90° (vertical head up); 2 instillation speeds, high (40 mL/min) and low (4 mL/min); and 2 different devices, a standard bulb-tipped gavage needle and an aerosolizing microsprayer designed for intratracheal instillation. One hour after treatment under these various conditions, rats were sacrificed, and the local distribution of the suspension in the lungs was observed. No animal restrained in the supine head-up or vertical head-up position died from the treatment; however, fatalities were observed when rats were restrained in the supine horizontal position except under high-speed dosing conditions with a microsprayer. Better distribution of the suspension in the lungs was observed in the rats restrained in the supine head-up position after instillation at high speed when compared with other conditions. These results indicated that high-speed instillation to the subject restrained in the supine head-up position is an appropriate condition for performing intratracheal instillation.
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