ArticleProceedings of the National Academy of Sciences of the United States of America2014
Circular permutation of a synthetic eukaryotic chromosome with the telomerator.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 42 citations in OpenAlex.
- Synthetic Chromosomes in Yeast: A Platform for Understanding and Programming Eukaryotic Life.Microbial biotechnology · 2026Review
- One Yeast, Sixteen Synthetic Chromosomes, Infinite Possibilities.Yeast (Chichester, England) · 2026Review
- Artificial chromosome reorganization reveals high plasticity of the budding and fission yeast genomes.Genome biology · 2025Article
- Methodological advances enabled by the construction of a synthetic yeast genome.Cell reports methods · 2024Article
- The synthetic future of algal genomes.Cell genomics · 2024Review
- Super-enhancers include classical enhancers and facilitators to fully activate gene expression.Cell · 2023Article
- Building synthetic chromosomes from natural DNA.Nature communications · 2023Article
- A supernumerary synthetic chromosome in Komagataella phaffii as a repository for extraneous genetic material.Microbial cell factories · 2023Article
- Synthetic chromosome fusion: Effects on mitotic and meiotic genome structure and function.Cell genomics · 2023Article
- Building synthetic chromosomes from natural DNA.bioRxiv : the preprint server for biology · 2023Article
- Construction of a synthetic Saccharomyces cerevisiae pan-genome neo-chromosome.Nature communications · 2022Article
- Rif1 regulates telomere length through conserved HEAT repeats.Nucleic acids research · 2021Article
- A supernumerary designer chromosome for modular in vivo pathway assembly in Saccharomyces cerevisiae.Nucleic acids research · 2021Article
- Sc3.0: revamping and minimizing the yeast genome.Genome biology · 2020Article
- Phylogenetic debugging of a complete human biosynthetic pathway transplanted into yeast.Nucleic acids research · 2020Article
- Creating a functional single-chromosome yeast.Nature · 2018Article
- Bug mapping and fitness testing of chemically synthesized chromosome X.Science (New York, N.Y.) · 2017Article
- Pathway swapping: Toward modular engineering of essential cellular processes.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chromosome engineering is a major focus in the fields of systems biology, genetics, synthetic biology, and the functional analysis of genomes. Here, we describe the "telomerator," a new synthetic biology device for use in Saccharomyces cerevisiae. The telomerator is designed to inducibly convert circular DNA molecules into mitotically stable, linear chromosomes replete with functional telomeres in vivo. The telomerator cassette encodes convergent yeast telomere seed sequences flanking the I-SceI homing endonuclease recognition site in the center of an intron artificially transplanted into the URA3 selectable/counterselectable auxotrophic marker. We show that inducible expression of the homing endonuclease efficiently generates linear molecules, identified by using a simple plate-based screening method. To showcase its functionality and utility, we use the telomerator to circularly permute a synthetic yeast chromosome originally constructed as a circular molecule, synIXR, to generate 51 linear variants. Many of the derived linear chromosomes confer unexpected phenotypic properties. This finding indicates that the telomerator offers a new way to study the effects of gene placement on chromosomes (i.e., telomere proximity). However, that the majority of synIXR linear derivatives support viability highlights inherent tolerance of S. cerevisiae to changes in gene order and overall chromosome structure. The telomerator serves as an important tool to construct artificial linear chromosomes in yeast; the concept can be extended to other eukaryotes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.