ArticlePlant physiology2015
Transgenic perturbation of the decarboxylation phase of Crassulacean acid metabolism alters physiology and metabolism but has only a small effect on growth.
Article in Plant physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 75 citations in OpenAlex.
- Integrative analysis of CAM photosynthesis reveals its impact on primary metabolism in Yucca.Journal of experimental botany · 2026Article
- Overexpression of cytosolic NADP-malic enzyme 1 from the common ice plant enhances water-deficit and high-light stress tolerance by modulating water-use efficiency and flavonoid biosynthesis.The Plant journal : for cell and molecular biology · 2026Article
- A PROMOTER::LUCIFERASE reporter system reveals key elements of the circadian regulation of Crassulacean acid metabolism (CAM) in Kalanchoë laxiflora Baker.The Plant journal : for cell and molecular biology · 2026Article
- MUTE drives asymmetric divisions to form stomatal subsidiary cells in Crassulaceae succulents.Science advances · 2026Article
- How evolution repeatedly builds complexity: a case study with CThe New phytologist · 2025Article
- Synthetic crassulacean acid metabolism (SynCAM) for improving water-use efficiency in plants.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025Review
- Regulation of Crassulacean acid metabolism at the protein level in Kalanchoë laxiflora.Plant physiology · 2025Article
- Bringing CAM photosynthesis to the table: Paving the way for resilient and productive agricultural systems in a changing climate.Plant communications · 2024Review
- Cucumber malate decarboxylase, CsNADP-ME2, functions in the balance of carbon and amino acid metabolism in fruit.Horticulture research · 2023Article
- Article
- Photosynthesis and leaf structure of F1 hybrids between Cymbidium ensifolium (C3) and C. bicolor subsp. pubescens (CAM).Annals of botany · 2023Article
- Elevated nocturnal respiratory rates in the mitochondria of CAM plants: current knowledge and unanswered questions.Annals of botany · 2023Article
- Developmental dynamics of crassulacean acid metabolism (CAM) in Opuntia ficus-indica.Annals of botany · 2023Article
- Prospects and perspectives: inferring physiological and regulatory targets for CAM from molecular and modelling approaches.Annals of botany · 2023Review
- Circadian regulation of metabolism across photosynthetic organisms.The Plant journal : for cell and molecular biology · 2023Review
- Evolution of Crassulacean acid metabolism in response to the environment: past, present, and future.Plant physiology · 2022Review
- CAM photosynthesis: the acid test.The New phytologist · 2022Article
- Article
- Phosphorolytic degradation of leaf starch via plastidic α-glucan phosphorylase leads to optimized plant growth and water use efficiency over the diel phases of Crassulacean acid metabolism.Journal of experimental botany · 2021Article
- Crassulacean acid metabolism species differ in the contribution of CPhysiologia plantarum · 2021Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Mitochondrial NAD-malic enzyme (ME) and/or cytosolic/plastidic NADP-ME combined with the cytosolic/plastidic pyruvate orthophosphate dikinase (PPDK) catalyze two key steps during light-period malate decarboxylation that underpin secondary CO(2) fixation in some Crassulacean acid metabolism (CAM) species. We report the generation and phenotypic characterization of transgenic RNA interference lines of the obligate CAM species Kalanchoë fedtschenkoi with reduced activities of NAD-ME or PPDK. Transgenic line rNAD-ME1 had 8%, and rPPDK1 had 5% of the wild-type level of activity, and showed dramatic changes in the light/dark cycle of CAM CO(2) fixation. In well-watered conditions, these lines fixed all of their CO(2) in the light; they thus performed C(3) photosynthesis. The alternative malate decarboxylase, NADP-ME, did not appear to compensate for the reduction in NAD-ME, suggesting that NAD-ME was the key decarboxylase for CAM. The activity of other CAM enzymes was reduced as a consequence of knocking out either NAD-ME or PPDK activity, particularly phosphoenolpyruvate carboxylase (PPC) and PPDK in rNAD-ME1. Furthermore, the circadian clock-controlled phosphorylation of PPC in the dark was reduced in both lines, especially in rNAD-ME1. This had the consequence that circadian rhythms of PPC phosphorylation, PPC kinase transcript levels and activity, and the classic circadian rhythm of CAM CO(2) fixation were lost, or dampened toward arrhythmia, under constant light and temperature conditions. Surprisingly, oscillations in the transcript abundance of core circadian clock genes also became arrhythmic in the rNAD-ME1 line, suggesting that perturbing CAM in K. fedtschenkoi feeds back to perturb the central circadian clock.
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