ArticlePlant & cell physiology2015
Whole-genome analysis of herbicide-tolerant mutant rice generated by Agrobacterium-mediated gene targeting.
Article in Plant & cell physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Heritable Tissue-Culture-Free Gene Editing in Nicotiana benthamiana through Viral Delivery of SpCas9 and sgRNA.Plant & cell physiology · 2024Article
- Influence of transgenesis on genome variability in cucumber lines with aPhysiology and molecular biology of plants : an international journal of functional plant biology · 2021Article
- Advantage of Nanotechnology-Based Genome Editing System and Its Application in Crop Improvement.Frontiers in plant science · 2021Review
- Potato Virus X Vector-Mediated DNA-Free Genome Editing in Plants.Plant & cell physiology · 2020Article
- Genomic variation between PRSV resistant transgenic SunUp and its progenitor cultivar Sunset.BMC genomics · 2020Article
- Fast-track breeding system to introduce CTV resistance of trifoliate orange into citrus germplasm, by integrating early flowering transgenic plants with marker-assisted selection.BMC plant biology · 2020Article
- The importance of genotype identity, genetic heterogeneity, and bioinformatic handling for properly assessing genomic variation in transgenic plants.BMC biotechnology · 2018Article
- Application of whole genome shotgun sequencing for detection and characterization of genetically modified organisms and derived products.Analytical and bioanalytical chemistry · 2016Review
- Genomic variation and DNA repair associated with soybean transgenesis: a comparison to cultivars and mutagenized plants.BMC biotechnology · 2016Article
- Transcriptome Profiling Revealed Stress-Induced and Disease Resistance Genes Up-Regulated in PRSV Resistant Transgenic Papaya.Frontiers in plant science · 2016Article
- Genome-wide profiling of genetic variation in Agrobacterium-transformed rice plants.Journal of Zhejiang University. Science. BArticle
- Frequency and type of inheritable mutations induced by γ rays in rice as revealed by whole genome sequencing.Journal of Zhejiang University. Science. BArticle
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Authors and funding
11 authors.
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Abstract
Gene targeting (GT) is a technique used to modify endogenous genes in target genomes precisely via homologous recombination (HR). Although GT plants are produced using genetic transformation techniques, if the difference between the endogenous and the modified gene is limited to point mutations, GT crops can be considered equivalent to non-genetically modified mutant crops generated by conventional mutagenesis techniques. However, it is difficult to guarantee the non-incorporation of DNA fragments from Agrobacterium in GT plants created by Agrobacterium-mediated GT despite screening with conventional Southern blot and/or PCR techniques. Here, we report a comprehensive analysis of herbicide-tolerant rice plants generated by inducing point mutations in the rice ALS gene via Agrobacterium-mediated GT. We performed genome comparative genomic hybridization (CGH) array analysis and whole-genome sequencing to evaluate the molecular composition of GT rice plants. Thus far, no integration of Agrobacterium-derived DNA fragments has been detected in GT rice plants. However, >1,000 single nucleotide polymorphisms (SNPs) and insertion/deletion (InDels) were found in GT plants. Among these mutations, 20-100 variants might have some effect on expression levels and/or protein function. Information about additive mutations should be useful in clearing out unwanted mutations by backcrossing.
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