Evidence map›Paper›PMID 25378653›Full record

Trial reportStem cells translational medicine2014

Optimized cell survival and seeding efficiency for craniofacial tissue engineering using clinical stem cell therapy.

Archana Rajan, Emily Eubanks, Sean Edwards, Sharon Aronovich, Suncica Travan, Ivan Rudek, Feng Wang, Alejandro Lanis, Darnell Kaigler

Open access · goldAbstract readClinical Trial
PubMed Full text at PMC Publisher
In one paragraph

Trial report in Stem cells translational medicine, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed, 1 pooled it
7.2field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 1 synthesis or guideline pooled it, 38 citations in OpenAlex.

  1. Pooled it
  2. Trial
  3. Craniofacial Fracture and Secretome: A Systematic Review.Plastic and reconstructive surgery. Global open · 2026
    Article
  4. Review
  5. Review
  6. Review
  7. Review
  8. Three-dimensional printing of clinical scale and personalized calcium phosphate scaffolds for alveolar bone reconstruction.Dental materials : official publication of the Academy of Dental Materials · 2022
    Article
  9. Review
  10. Article
  11. Review
  12. Article
  13. Review
  14. Review
  15. Review
  16. Review
  17. Bone Marrow Stromal Stem Cells in Tissue Engineering and Regenerative Medicine.Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme · 2016
    Review
  18. Review
  19. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Archana RajanDepartment of Orthodontics and Pediatric Dentistry, Department of Periodontics and Oral Medicine, Department of Oral and Maxillofacial Surgery, Center for Oral Health Research, and Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan, USA.
Emily EubanksDepartment of Orthodontics and Pediatric Dentistry, Department of Periodontics and Oral Medicine, Department of Oral and Maxillofacial Surgery, Center for Oral Health Research, and Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan, USA.
Sean EdwardsDepartment of Orthodontics and Pediatric Dentistry, Department of Periodontics and Oral Medicine, Department of Oral and Maxillofacial Surgery, Center for Oral Health Research, and Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan, USA.
Sharon AronovichDepartment of Orthodontics and Pediatric Dentistry, Department of Periodontics and Oral Medicine, Department of Oral and Maxillofacial Surgery, Center for Oral Health Research, and Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan, USA.
Suncica TravanDepartment of Orthodontics and Pediatric Dentistry, Department of Periodontics and Oral Medicine, Department of Oral and Maxillofacial Surgery, Center for Oral Health Research, and Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan, USA.
Ivan RudekDepartment of Orthodontics and Pediatric Dentistry, Department of Periodontics and Oral Medicine, Department of Oral and Maxillofacial Surgery, Center for Oral Health Research, and Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan, USA.
Feng WangDepartment of Orthodontics and Pediatric Dentistry, Department of Periodontics and Oral Medicine, Department of Oral and Maxillofacial Surgery, Center for Oral Health Research, and Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan, USA.
Alejandro LanisDepartment of Orthodontics and Pediatric Dentistry, Department of Periodontics and Oral Medicine, Department of Oral and Maxillofacial Surgery, Center for Oral Health Research, and Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan, USA.
Darnell KaiglerDepartment of Orthodontics and Pediatric Dentistry, Department of Periodontics and Oral Medicine, Department of Oral and Maxillofacial Surgery, Center for Oral Health Research, and Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan, USA dkaigler@umich.edu.
University of Michigan–Ann Arbor · US

Funding

Michigan Institute for Clinical and Health Research (MCHR)UL1TR000433 · NCATS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI MASHOUR, GEORGE ALEXANDER · 2012 to 2016
$49.9M
Alveolar Bone Marrow Derived Stem Cells for Clinical Cell TherapyR56DE023095 · NIDCR · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI KAIGLER, DARNELL · 2013 to 2013
$311k
NCATS NIH HHS UL1 TR000433NIDCR NIH HHS R56 DE023095
6 · The paper itself

Abstract

Traumatic injuries involving the face are very common, yet the clinical management of the resulting craniofacial deficiencies is challenging. These injuries are commonly associated with missing teeth, for which replacement is compromised due to inadequate jawbone support. Using cell therapy, we report the upper jaw reconstruction of a patient who lost teeth and 75% of the supporting jawbone following injury. A mixed population of bone marrow-derived autologous stem and progenitor cells was seeded onto β-tricalcium phosphate (β-TCP), which served as a scaffold to deliver cells directly to the defect. Conditions (temperature, incubation time) to achieve the highest cell survival and seeding efficiency were optimized. Four months after cell therapy, cone beam computed tomography and a bone biopsy were performed, and oral implants were placed to support an engineered dental prosthesis. Cell seeding efficiency (>81%) of the β-TCP and survival during the seeding process (94%) were highest when cells were incubated with β-TCP for 30 minutes, regardless of incubation temperature; however, at 1 hour, cell survival was highest when incubated at 4°C. Clinical, radiographic, and histological analyses confirmed that by 4 months, the cell therapy regenerated 80% of the original jawbone deficiency with vascularized, mineralized bone sufficient to stably place oral implants. Functional and aesthetic rehabilitation of the patient was successfully completed with installation of a dental prosthesis 6 months following implant placement. This proof-of-concept clinical report used an evidence-based approach for the cell transplantation protocol used and is the first to describe a cell therapy for craniofacial trauma reconstruction.

Indexed as

BioprosthesisBone Marrow CellsBone Marrow TransplantationDental ProsthesisMaxillaOsteogenesisTissue EngineeringTissue ScaffoldsAutograftsCalcium PhosphatesCell- and Tissue-Based TherapyCell SurvivalFemaleHumansMiddle AgedRadiographybeta-tricalcium phosphateCalcium PhosphatesBone marrowBone regenerationCell therapyImplantsScaffoldStem cells

Identifiers

PMID25378653
PMCPMC4250207
OpenAlexW2071103725

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.