ArticleThe Journal of cell biology1989
A Dictyostelium mutant deficient in severin, an F-actin fragmenting protein, shows normal motility and chemotaxis.
Article in The Journal of cell biology, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 98 citations in OpenAlex.
- The excitable signal transduction networks: movers and shapers of eukaryotic cell migration.The International journal of developmental biology · 2019Review
- A Simple Retroelement Based Knock-Down System in Dictyostelium: Further Insights into RNA Interference Mechanisms.PloS one · 2015Article
- Arp2/3 complex-deficient mouse fibroblasts are viable and have normal leading-edge actin structure and function.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Tortoise, a novel mitochondrial protein, is required for directional responses of Dictyostelium in chemotactic gradients.The Journal of cell biology · 2001Article
- How well can an amoeba climb?Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Gelsolin deficiency blocks podosome assembly and produces increased bone mass and strength.The Journal of cell biology · 2000Article
- Capping protein terminates but does not initiate chemoattractant-induced actin assembly in Dictyostelium.The Journal of cell biology · 1997Article
- Xenopus actin depolymerizing factor/cofilin (XAC) is responsible for the turnover of actin filaments in Listeria monocytogenes tails.The Journal of cell biology · 1997Article
- Interaction of a Dictyostelium member of the plastin/fimbrin family with actin filaments and actin-myosin complexes.Molecular biology of the cell · 1997Article
- Actin organization, bristle morphology, and viability are affected by actin capping protein mutations in Drosophila.The Journal of cell biology · 1996Article
- Mechanical perturbation elicits a phenotypic difference between Dictyostelium wild-type cells and cytoskeletal mutants.Biophysical journal · 1996Article
- Identification of a cyclase-associated protein (CAP) homologue in Dictyostelium discoideum and characterization of its interaction with actin.Molecular biology of the cell · 1996Article
- Actin filament barbed-end capping activity in neutrophil lysates: the role of capping protein-beta 2.Molecular biology of the cell · 1995Article
- Molecular and mutational analysis of a gelsolin-family member encoded by the flightless I gene of Drosophila melanogaster.Genetics · 1995Article
- A talin homologue of Dictyostelium rapidly assembles at the leading edge of cells in response to chemoattractant.The Journal of cell biology · 1995Article
- Cell-substrate interactions and locomotion of Dictyostelium wild-type and mutants defective in three cytoskeletal proteins: a study using quantitative reflection interference contrast microscopy.Biophysical journal · 1995Article
- Ponticulin is the major high affinity link between the plasma membrane and the cortical actin network in Dictyostelium.The Journal of cell biology · 1994Article
- Dictyostelium discoideum mutants with conditional defects in phagocytosis.The Journal of cell biology · 1994Article
- Identification of secreted and cytosolic gelsolin in Drosophila.The Journal of cell biology · 1994Article
- Replacement of the phospholipid-anchor in the contact site A glycoprotein of D. discoideum by a transmembrane region does not impede cell adhesion but reduces residence time on the cell surface.The Journal of cell biology · 1994Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A severin deficient mutant of Dictyostelium discoideum has been isolated by the use of colony immunoblotting after chemical mutagenesis. In homogenates of wild-type cells, severin is easily detected as a very active F-actin fragmenting protein. Tests for severin in the mutant, HG1132, included viscometry for the assay of F-actin fragmentation in fractions from DEAE-cellulose columns, labeling of blots with monoclonal and polyclonal antibodies, and immunofluorescent-labeling of cryosections. Severin could not be detected in the mutant using these methods. The mutation in HG1132 is recessive and has been mapped to linkage group VII. The mutant failed to produce the normal severin mRNA, but small amounts of a transcript that was approximately 100 bases larger than the wild-type mRNA were detected in the mutant throughout all stages of development. On the DNA level a new Mbo II restriction site was found in the mutant within the coding region of the severin gene. The severin deficient mutant cells grew at an approximately normal rate, aggregated and formed fruiting bodies with viable spores. By the use of an image processing system, speed of cell movement, turning rates, and precision of chemotactic orientation in a stable gradient of cyclic AMP were quantitated, and no significant differences between wild-type and mutant cells were found. Thus, under the culture conditions used, severin proved to be neither essential for growth of D. discoideum nor for any cell function that is important for aggregation or later development.
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