ArticleThe Journal of biological chemistry2014
Nitroxyl (HNO) reacts with molecular oxygen and forms peroxynitrite at physiological pH. Biological Implications.
Article in The Journal of biological chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed.
- Real-time photoactivated SRS imaging enables spatiotemporally controlled nitroxyl release and therapeutic guidance in vivo.Nature communications · 2026Article
- Toward Specific Detection of Peroxynitrite─Intramolecular Cyclization of Boronobenzylated Pyridinium Probe in Chemical and Cellular Systems.Chemical research in toxicology · 2026Article
- Reactive Nitrogen Species and Fibrinogen: Exploring the Effects of Nitration on Blood Clots.Antioxidants (Basel, Switzerland) · 2025Review
- Nitrous oxide production via enzymatic nitroxyl from the nitrifying archaeonProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Boronate-Based Bioactive Compounds Activated by Peroxynitrite and Hydrogen Peroxide.Redox biochemistry and chemistry · 2024Article
- Small molecule probes for peroxynitrite detection.Redox biochemistry and chemistry · 2024Article
- Determination of Nitric Oxide and Its Metabolites in Biological Tissues Using Ozone-Based Chemiluminescence Detection: A State-of-the-Art Review.Antioxidants (Basel, Switzerland) · 2024Review
- Closing Dichloramine Decomposition Nitrogen and Oxygen Mass Balances: Relative Importance of End-Products from the Reactive Nitrogen Species Pathway.Environmental science & technology · 2024Article
- C-Nitrosothioformamide: A Donor Template for Dual Release of HNO and HChembiochem : a European journal of chemical biology · 2022Article
- HNO Protects the Myocardium against Reperfusion Injury, Inhibiting the mPTP Opening via PKCε Activation.Antioxidants (Basel, Switzerland) · 2022Article
- The Chemistry of HNO: Mechanisms and Reaction Kinetics.Frontiers in chemistry · 2022Review
- Kinetics of Azanone (HNO) Reactions with Thiols: Effect of pH.Cell biochemistry and biophysics · 2021Article
- Kinetic Study on the Reactivity of Azanone (HNO) toward CyclicInternational journal of molecular sciences · 2021Article
- Azanone (HNO): generation, stabilization and detection.Chemical science · 2021Review
- Redox and Antioxidant Modulation of Circadian Rhythms: Effects of Nitroxyl, N-Acetylcysteine and Glutathione.Molecules (Basel, Switzerland) · 2021Article
- Updated Reaction Pathway for Dichloramine Decomposition: Formation of Reactive Nitrogen Species andEnvironmental science & technology · 2021Article
- Tracking isotopically labeled oxidants using boronate-based redox probes.The Journal of biological chemistry · 2020Article
- Boronate-Based Probes for Biological Oxidants: A Novel Class of Molecular Tools for Redox Biology.Frontiers in chemistry · 2020Review
- Detection and quantification of nitric oxide-derived oxidants in biological systems.The Journal of biological chemistry · 2019Review
- A Chemiluminescent Probe for HNO Quantification and Real-Time Monitoring in Living Cells.Angewandte Chemie (International ed. in English) · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
Nitroxyl (HNO), the protonated one-electron reduction product of NO, remains an enigmatic reactive nitrogen species. Its chemical reactivity and biological activity are still not completely understood. HNO donors show biological effects different from NO donors. Although HNO reactivity with molecular oxygen is described in the literature, the product of this reaction has not yet been unambiguously identified. Here we report that the decomposition of HNO donors under aerobic conditions in aqueous solutions at physiological pH leads to the formation of peroxynitrite (ONOO(-)) as a major intermediate. We have specifically detected and quantified ONOO(-) with the aid of boronate probes, e.g. coumarin-7-boronic acid or 4-boronobenzyl derivative of fluorescein methyl ester. In addition to the major phenolic products, peroxynitrite-specific minor products of oxidation of boronate probes were detected under these conditions. Using the competition kinetics method and a set of HNO scavengers, the value of the second order rate constant of the HNO reaction with oxygen (k = 1.8 × 10(4) m(-1) s(-1)) was determined. The rate constant (k = 2 × 10(4) m(-1) s(-1)) was also determined using kinetic simulations. The kinetic parameters of the reactions of HNO with selected thiols, including cysteine, dithiothreitol, N-acetylcysteine, captopril, bovine and human serum albumins, and hydrogen sulfide, are reported. Biological and cardiovascular implications of nitroxyl reactions are discussed.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.