ArticleScientific reports2014
Understanding of how Propionibacterium acidipropionici respond to propionic acid stress at the level of proteomics.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 53 citations in OpenAlex.
- A transcriptomic atlas of acute stress response to low pH in multipleMicrobiology spectrum · 2024Article
- The Universally Conserved Unconventional G Protein YchF Is Critical for Growth and Stress Response.Life (Basel, Switzerland) · 2023Review
- Understanding the Streptomyces albulus response to low-pH stress at the interface of physiology and transcriptomics.Applied microbiology and biotechnology · 2023Article
- Exploring the Extreme Acid Tolerance of a Dynamic Protein Nanocage.Biomacromolecules · 2023Article
- Growth on Formic Acid Is Dependent on Intracellular pH Homeostasis for the Thermoacidophilic MethanotrophFrontiers in microbiology · 2021Article
- Propionic Acid: Method of Production, Current State and Perspectives.Food technology and biotechnology · 2020Review
- Sequencing and Analysis of the Genome ofBiomolecules · 2020Article
- Microbial response to acid stress: mechanisms and applications.Applied microbiology and biotechnology · 2020Review
- Transcriptomics and Proteomics Analyses of the Responses ofFrontiers in microbiology · 2020Article
- Stress induced biofilm formation in Propionibacterium acidipropionici and use in propionic acid production.World journal of microbiology & biotechnology · 2019Article
- Propionibacterium spp.-source of propionic acid, vitamin B12, and other metabolites important for the industry.Applied microbiology and biotechnology · 2018Review
- Determinants of selection in yeast evolved by genome shuffling.Biotechnology for biofuels · 2018Article
- Transient MutS-Based Hypermutation System for Adaptive Evolution of Lactobacillus casei to Low pH.Applied and environmental microbiology · 2017Article
- Dairy Propionibacteria: Versatile Probiotics.Microorganisms · 2017Review
- Article
- Long-term adaptive evolution ofBiotechnology for biofuels · 2016Article
- Genome shuffling of the nonconventional yeast Pichia anomala for improved sugar alcohol production.Microbial cell factories · 2015Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Propionic acid (PA) is an important platform chemical in the food, agriculture, and pharmaceutical industries and is mainly biosynthesized by propionibacteria. Acid tolerance in PA-producing strains is crucial. In previous work, we investigated the acid tolerance mechanism of Propionibacterium acidipropionici at microenvironmental levels by analyzing physiological changes in the parental strain and three PA-tolerant mutants obtained by genome shuffling. However, the molecular mechanism of PA tolerance in P. acidipropionici remained unclear. Here, we performed a comparative proteomics study of P. acidipropionici CGMCC 1.2230 and the acid-tolerant mutant P. acidipropionici WSH1105; MALDI-TOF/MS identified 24 proteins that significantly differed between the parental and shuffled strains. The differentially expressed proteins were mainly categorized as key components of crucial biological processes and the acid stress response. Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) was used to confirm differential expression of nine key proteins. Overexpression of the secretory protein glyceraldehyde-3-phosphate dehydrogenase and ATP synthase subunit α in Escherichia coli BL21 improved PA and acetic acid tolerance; overexpression of NADH dehydrogenase and methylmalonyl-CoA epimerase improved PA tolerance. These results provide new insights into the acid tolerance of P. acidipropionici and will facilitate the development of PA production through fermentation by propionibacteria.
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