ArticleGraefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie2014
Hyalocytes in idiopathic epiretinal membranes: a correlative light and electron microscopic study.
Article in Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 60 citations in OpenAlex.
- Chromovitrectomy: current evidence, emerging technologies, and practical applications in vitreoretinal surgery.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2026Review
- The existence of puckering in the retina beyond the macula: a prospective analysis.International ophthalmology · 2026Article
- Differentiation of human hyalocytes from induced pluripotent stem cells through ascorbic acid treatment.Human cell · 2025Article
- Epiretinal Membrane: Correlations Among Clinical, Immunohistochemical, and Biochemical Features and Their Prognostic Implications.Investigative ophthalmology & visual science · 2024Article
- Hyalocytes-guardians of the vitreoretinal interface.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2024Review
- Aqueous Humor Cytokines in Idiopathic Epiretinal Membrane: Correlation with Disease Severity.Diagnostics (Basel, Switzerland) · 2024Article
- [Epiretinal membrane: diagnostics, indications and surgical treatment].Die Ophthalmologie · 2024Review
- Short-term-outcomes of idiopathic epiretinal membranes treated with pars-plana-vitrectomy - examination of visual function and OCT-morphology.International journal of retina and vitreous · 2023Article
- Co-Expression of Podoplanin and CD44 in Proliferative Vitreoretinopathy Epiretinal Membranes.International journal of molecular sciences · 2023Review
- 3-D OCT imaging of hyalocytes in partial posterior vitreous detachment and vaso-occlusive retinal disease.American journal of ophthalmology case reports · 2023Article
- Pathophysiology and clinical aspects of epiretinal membrane - review.Frontiers in medicine · 2023Review
- Review
- Cellular components of the idiopathic epiretinal membrane.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2022Review
- Imaging of vitreous cortex hyalocyte dynamics using non-confocal quadrant-detection adaptive optics scanning light ophthalmoscopy in human subjects.Biomedical optics express · 2022Article
- Hyalocytes in proliferative vitreo-retinal diseases.Expert review of ophthalmology · 2022Article
- The extracellular matrix complexity of idiopathic epiretinal membranes and the bilaminar arrangement of the associated internal limiting membrane in the posterior retina.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2021Article
- En Face Optical Coherence Tomography Imaging of Foveal Dots in Eyes With Posterior Vitreous Detachment or Internal Limiting Membrane Peeling.Investigative ophthalmology & visual science · 2021Article
- [Statement of the Professional Association of German Ophthalmologists (BVA), the German Ophthalmological Society (DOG) and the Retinological Society (RG) on the development, diagnostics and treatment of epiretinal gliosis : Status October 2020].Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft · 2021Review
- Epiretinal membrane surgery outcome in eyes with abnormalities of the central bouquet.International journal of retina and vitreous · 2021Article
- Heat Shock Protein 90 Involvement in the Development of Idiopathic Epiretinal Membranes.Investigative ophthalmology & visual science · 2020Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeTo describe characteristics of epiretinal cells at the vitreoretinal interface by correlative light and electron microscopy (CLEM).
methodsEpiretinal membrane (ERM) specimens and internal limiting membrane (ILM) specimens were harvested by sequential peeling during vitrectomy from 27 eyes with idiopathic epiretinal gliosis, and processed for CLEM. Intraoperatively, the presence of posterior vitreous detachment (PVD) was documented. We used anti-vimentin, anti-α-smooth muscle actin (α-SMA), and anti-CD45 as primary antibodies. A fluorescein-tagged immunonanogold cluster was used as secondary antibody and visualized under the fluorescence and transmission electron microscope.
resultsWe demonstrated CD45-positive cells specifically labelled at their plasma membranes with ultrastructural features known for hyalocytes, such as oval nucleus with marginal chromatin, vacuoles, dense granules, and thin cytoplasmic protrusions. CD45-positive cells were mostly located on a thick layer of native vitreous collagen. They were covered by newly formed collagen strands with multilayered proliferation of myofibroblasts. We also demonstrated immunoreactivity for vimentin and alpha-SMA. Cell fragments with positive labelling for α-SMA and vimentin were not only found on the vitreal side of the ILM, but also on the retinal side.
conclusionsBy CLEM, the majority of CD45-positive cells in epiretinal cell proliferation were characterized as hyalocytes. In the context of anomalous PVD and vitreoschisis, ultrastructural features and topographic localization of hyalocytes suggest that these cells play a significant role in ERM formation. CLEM enables a more accurate characterization of epiretinal cell proliferation, and therefore, contributes to a better understanding of the pathogenesis of diseases at the vitreoretinal interface.
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