ArticleOecologia2015
Direct determination of fatty acids in fish tissues: quantifying top predator trophic connections.
Article in Oecologia, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 85 citations in OpenAlex.
- Fatty Acid Profiling of Fish Fin Tissues: Implications for Non-Lethal Sampling Methods.Ecology and evolution · 2026Article
- Upcycled Animal Protein Hydrolysates for Gilthead Seabream: Impact on Growth Performance, Nutrient Utilisation, Intestinal Structure and Skeletal Muscle Gene Expression.Aquaculture nutrition · 2026Article
- Resource use of dusky and Galapagos sharks in response to fishing activities at a remote Pacific Island.Scientific reports · 2025Article
- Production, Transport, Fate and Effects of Lipids in the Marine Environment.Marine drugs · 2025Review
- Locally-Sourced Animal Protein Hydrolysates in High-Plant-Protein Diets Can Promote European Seabass Growth and Nutrient Utilization, Reducing Reliance on Fishmeal.Aquaculture nutrition · 2025Article
- Cryopreservation of six Symbiodiniaceae genera and assessment of fatty acid profiles in response to increased salinity treatments.Scientific reports · 2022Article
- The Comparison of Fatty Acid Composition and Lipid Quality Indices of Roach, Perch, and Pike of Lake Gusinoe (Western Transbaikalia).International journal of environmental research and public health · 2021Article
- Physiological markers suggest energetic and nutritional adjustments in male sharks linked to reproduction.Oecologia · 2021Article
- Uncovering the sub-lethal impacts of plastic ingestion by shearwaters using fatty acid analysis.Conservation physiology · 2019Article
- Lipid and fatty acid dynamics in mature female albacore tuna (Thunnus alalunga) in the western Indian Ocean.PloS one · 2018Article
- Spatial Patterns and Temperature Predictions of Tuna Fatty Acids: Tracing Essential Nutrients and Changes in Primary Producers.PloS one · 2015Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fatty acids are a valuable tool in ecological studies because of the large number of unique structures synthesized. They provide versatile signatures that are being increasingly employed to delineate the transfer of dietary material through marine and terrestrial food webs. The standard procedure for determining fatty acids generally involves lipid extraction followed by methanolysis to produce methyl esters for analysis by gas chromatography. By directly transmethylating ~50 mg wet samples and adding an internal standard it was possible to greatly simplify the analytical methodology to enable rapid throughput of 20-40 fish tissue fatty acid analyses a day including instrumental analysis. This method was verified against the more traditional lipid methods using albacore tuna and great white shark muscle and liver samples, and it was shown to provide an estimate of sample dry mass, total lipid content, and a condition index. When large fatty acid data sets are generated in this way, multidimensional scaling, analysis of similarities, and similarity of percentages analysis can be used to define trophic connections among samples and to quantify them. These routines were used on albacore and skipjack tuna fatty acid data obtained by direct methylation coupled with literature values for krill. There were clear differences in fatty acid profiles among the species as well as spatial differences among albacore tuna sampled from different locations.
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Registered trials
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