ArticleNature protocols2014
Anesthetized- and awake-patched whole-cell recordings in freely moving rats using UV-cured collar-based electrode stabilization.
Article in Nature protocols, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 27 citations in OpenAlex.
- A cranial implant for stabilizing whole-cell patch-clamp recordings in behaving rodents.Journal of neuroscience methods · 2023Article
- Concurrent recordings of hippocampal neuronal spikes and prefrontal synaptic inputs from an awake rat.STAR protocols · 2021Article
- Anesthetics fragment hippocampal network activity, alter spine dynamics, and affect memory consolidation.PLoS biology · 2021Article
- In Vivo Whole-Cell Patch-Clamp Methods: Recent Technical Progress and Future Perspectives.Sensors (Basel, Switzerland) · 2021Review
- Intracellular neuronal recording in awake nonhuman primates.Nature protocols · 2020Article
- Optimal Pipette Resistance, Seal Resistance, and Zero-Current Membrane Potential for Loose Patch or Breakthrough Whole-Cell RecordingFrontiers in neural circuits · 2020Article
- Pharmaco-electroencephalographic responses in the rat differ between active and inactive locomotor states.The European journal of neuroscience · 2019Article
- Subthreshold Activity Underlying the Diversity and Selectivity of the Primary Auditory Cortex Studied by Intracellular Recordings in Awake Marmosets.Cerebral cortex (New York, N.Y. : 1991) · 2019Article
- Robotic navigation to subcortical neural tissue for intracellular electrophysiology in vivo.Journal of neurophysiology · 2017Article
- Experience-dependent shaping of hippocampal CA1 intracellular activity in novel and familiar environments.eLife · 2017Article
- Detachable glass microelectrodes for recording action potentials in active moving organs.American journal of physiology. Heart and circulatory physiology · 2017Article
- In vivo whole-cell recording with high success rate in anaesthetized and awake mammalian brains.Molecular brain · 2016Article
- Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Intracellular recording allows precise measurement and manipulation of individual neurons, but it requires stable mechanical contact between the electrode and the cell membrane, and thus it has remained challenging to perform in behaving animals. Whole-cell recordings in freely moving animals can be obtained by rigidly fixing ('anchoring') the pipette electrode to the head; however, previous anchoring procedures were slow and often caused substantial pipette movement, resulting in loss of the recording or of recording quality. We describe a UV-transparent collar and UV-cured adhesive technique that rapidly (within 15 s) anchors pipettes in place with virtually no movement, thus substantially improving the reliability, yield and quality of freely moving whole-cell recordings. Recordings are first obtained from anesthetized or awake head-fixed rats. UV light cures the thin adhesive layers linking pipette to collar to head. Then, the animals are rapidly and smoothly released for recording during unrestrained behavior. The anesthetized-patched version can be completed in ∼4-7 h (excluding histology) and the awake-patched version requires ∼1-4 h per day for ∼2 weeks. These advances should greatly facilitate studies of neuronal integration and plasticity in identified cells during natural behaviors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.