ArticleNature protocols2014
Production and analysis of immunomodulatory excretory-secretory products from the mouse gastrointestinal nematode Heligmosomoides polygyrus bakeri.
Article in Nature protocols, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 39 citations in OpenAlex.
- From infection to cholangiocarcinoma: Why opisthorchiids break these rules.One health (Amsterdam, Netherlands) · 2026Review
- In vivo reduction of Treg expansion in rodent helminth-malaria coinfection.Parasites & vectors · 2026Article
- Characterization and ligand binding properties of a fatty acid- and retinol- binding protein (Hp-FAR-2) from Heligmosomoides polygyrus.PLoS neglected tropical diseases · 2025Article
- A secreted fatty acid- and retinol- binding protein frombioRxiv : the preprint server for biology · 2025Article
- Maternal gastrointestinal nematode infection alters hippocampal neuroimmunity, promotes synaptic plasticity, and improves resistance to direct infection in offspring.Scientific reports · 2024Article
- Helminth-induced reprogramming of the stem cell compartment inhibits type 2 immunity.The Journal of experimental medicine · 2022Article
- Maternal gastrointestinal nematode infection enhances spatial memory of uninfected juvenile mouse pups.Scientific reports · 2022Article
- Characterization of a novel cysteine protease inhibitor inFrontiers in immunology · 2022Article
- Immunomodulatory action of excretory-secretory products of Angiostrongylus cantonensis in a mouse tumour model.Parasitology research · 2020Article
- Proteomic analysis revealed T cell hyporesponsiveness induced by Haemonchus contortus excretory and secretory proteins.Veterinary research · 2020Article
- Article
- Age affects antibody levels and anthelmintic treatment efficacy in a wild rodent.International journal for parasitology. Parasites and wildlife · 2019Article
- Analysis of the Trichuris suis excretory/secretory proteins as a function of life cycle stage and their immunomodulatory properties.Scientific reports · 2018Article
- Of dogs and hookworms: man's best friend and his parasites as a model for translational biomedical research.Parasites & vectors · 2018Review
- Host-Parasite Interactions Promote Disease Tolerance to Intestinal Helminth Infection.Frontiers in immunology · 2018Review
- Epithelial-Cell-Derived Phospholipase ACell host & microbe · 2017Article
- IRF-8 regulates expansion of myeloid-derived suppressor cells and Foxp3+ regulatory T cells and modulates Th2 immune responses to gastrointestinal nematode infection.PLoS pathogens · 2017Article
- Primary Heligmosomoides polygyrus bakeri infection induces myeloid-derived suppressor cells that suppress CD4Mucosal immunology · 2017Article
- Article
- Downregulation of the Syk Signaling Pathway in Intestinal Dendritic Cells Is Sufficient To Induce Dendritic Cells That Inhibit Colitis.Journal of immunology (Baltimore, Md. : 1950) · 2016Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Heligmosomoides polygyrus bakeri (Hpb) infection in mice is a convenient model for studying the pathophysiology and immunology of gastrointestinal (GI) helminth infection. Hpb infection suppresses immune responses to bystander antigens and unrelated pathogens, and it slows the progression and modifies the outcome of immune-mediated diseases. Hpb-derived excretory-secretory (ES) products potently modulate CD4(+) helper T cell (TH) responses by inducing regulatory T cells, tolerogenic dendritic cells (DCs) and immunoregulatory cytokines. This observation has spiked interest in identifying the immunomodulatory molecules, especially proteins, in ES products from Hpb and other GI nematodes for development as novel therapies to treat individuals with immune-mediated diseases, such as inflammatory bowel diseases (IBDs). In this protocol, we describe how to (i) maintain Hpb in the laboratory for experimental infections, (ii) collect adult worms from infected mice to generate ES products and (iii) evaluate the modulatory effects of ES products on toll-like receptor (TLR) ligand-induced maturation of CD11c(+) DCs. The three major sections of the PROCEDURE can be used independently, and they require ∼6, 10 and 27 h, respectively. Although other methods use a modified Baermann apparatus to collect Hpb adult worms, we describe a method that involves dissection of adult worms from intestinal tissue. The protocol will be useful to investigators studying the host-parasite interface and identifying and analyzing helminth-derived molecules with therapeutic potential.
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