ArticlePloS one2014
Differential effects of tissue culture coating substrates on prostate cancer cell adherence, morphology and behavior.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
37 citing papers in PubMed, 89 citations in OpenAlex.
- Article
- Periprostatic adipose tissue-derived extracellular vesicles modulate prostate cancer cell behaviour in vitro according to tumour grade.Molecular medicine (Cambridge, Mass.) · 2026Article
- Polydopamine-Coated Surfaces Promote Adhesion, Migration, Proliferation, Chemoresistance, Stemness, and Epithelial-Mesenchymal Transition of Human Prostate Cancer Cell Lines In Vitro via Integrin αInternational journal of molecular sciences · 2026Article
- Microfluidic Applications in Prostate Cancer Research.Micromachines · 2024Review
- Enhancing Nasopharyngeal Carcinoma Cell Separation with Selective Fibronectin Coating and Topographical Modification on Polydimethylsiloxane Scaffold Platforms.International journal of molecular sciences · 2023Article
- Metasurface-enhanced infrared spectroscopy in multiwell format for real-time assaying of live cells.Lab on a chip · 2023Article
- Microfluidic Wound-Healing Assay for ECM and Microenvironment Properties on Microglia BV2 Cells Migration.Biosensors · 2023Article
- Achieving Single-Molecule Tracking of Subcellular Regulation in Bacteria during Real-Time Environmental Perturbations.Analytical chemistry · 2023Article
- See-N-Seq: RNA sequencing of target single cells identified by microscopy via micropatterning of hydrogel porosity.Communications biology · 2022Article
- Decellularised Cartilage ECM Culture Coatings Drive Rapid and Robust Chondrogenic Differentiation of Human Periosteal Cells.Bioengineering (Basel, Switzerland) · 2022Article
- In vitro cultivation of primary intestinal cells from Eisenia fetida as basis for ecotoxicological studies.Ecotoxicology (London, England) · 2022Article
- Prostate cancer cells of increasing metastatic potential exhibit diverse contractile forces, cell stiffness, and motility in a microenvironment stiffness-dependent manner.Frontiers in cell and developmental biology · 2022Article
- Direct addition of poly-lysine or poly-ethylenimine to the medium: A simple alternative to plate pre-coating.PloS one · 2022Article
- Double Labeling Fluorescent Immunocytochemistry.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Human nasal septal chondrocytes (NSCs) preconditioned on NSC-derived matrix improve their chondrogenic potential.Biomaterials research · 2021Article
- Bioinformatics and Functional Analyses Implicate Potential Roles for EOGT and L-fringe in Pancreatic Cancers.Molecules (Basel, Switzerland) · 2021Article
- Protein Substrate Alters Cell Physiology in Primary Culture of Vocal Fold Epithelial Cells.Cells, tissues, organs · 2021Article
- PD-L1 Influences Cell Spreading, Migration and Invasion in Head and Neck Cancer Cells.International journal of molecular sciences · 2020Article
- A Sample Preparation Protocol for High Throughput Immunofluorescence of Suspension Cells on an Adherent Surface.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2020Article
- Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Weak cell-surface adhesion of cell lines to tissue culture surfaces is a common problem and presents technical limitations to the design of experiments. To overcome this problem, various surface coating protocols have been developed. However, a comparative and precise real-time measurement of their impact on cell behavior has not been conducted. The prostate cancer cell line LNCaP, derived from a patient lymph node metastasis, is a commonly used model system in prostate cancer research. However, the cells' characteristically weak attachment to the surface of tissue culture vessels and cover slips has impeded their manipulation and analysis and use in high throughput screening. To improve the adherence of LNCaP cells to the culture surface, we compared different coating reagents (poly-L-lysine, poly-L-ornithine, collagen type IV, fibronectin, and laminin) and culturing conditions and analyzed their impact on cell proliferation, adhesion, morphology, mobility and gene expression using real-time technologies. The results showed that fibronectin, poly-L-lysine and poly-L-ornithine improved LNCaP cells adherence and provoked cell morphology alterations, such as increase of nuclear and cellular area. These coating reagents also induced a higher expression of F-actin and reduced cell mobility. In contrast, laminin and collagen type IV did not improve adherence but promoted cell aggregation and affected cell morphology. Cells cultured in the presence of laminin displayed higher mobility than control cells. All the coating conditions significantly affected cell viability; however, they did not affect the expression of androgen receptor-regulated genes. Our comparative findings provide important insight for the selection of the ideal coating reagent and culture conditions for the cancer cell lines with respect to their effect on proliferation rate, attachment, morphology, migration, transcriptional response and cellular cytoskeleton arrangement.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.