ArticlePloS one2014
A novel modular bioreactor to in vitro study the hepatic sinusoid.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Engineering biomimetic tissue barrier models on chips: From design and fabrication to applications in disease modeling and drug screening.Biomaterials · 2026Review
- Vasomics of the liver.Gut · 2025Review
- Mechanobiology of portal hypertension.JHEP reports : innovation in hepatology · 2023Review
- Openable artificial intestinal tract device integrated with a permeable filter for evaluating drug permeation through cells.Scientific reports · 2023Article
- Biomechanics in liver regeneration after partial hepatectomy.Frontiers in bioengineering and biotechnology · 2023Review
- Physiologically relevant microsystems to study viral infection in the human liver.Frontiers in microbiology · 2022Review
- Influence of microflow on hepatic sinusoid blood flow and red blood cell deformation.Biophysical journal · 2021Article
- Liver Bioreactor Design Issues of Fluid Flow and Zonation, Fibrosis, and Mechanics: A Computational Perspective.Journal of functional biomaterials · 2020Review
- Hepatic microcirculation and mechanisms of portal hypertension.Nature reviews. Gastroenterology & hepatology · 2019Review
- Resemblance of the human liver sinusoid in a fluidic device with biomedical and pharmaceutical applications.Biotechnology and bioengineering · 2018Article
- Flat and microstructured polymeric membranes in organs-on-chips.Journal of the Royal Society, Interface · 2018Review
- Engineering and monitoring cellular barrier models.Journal of biological engineering · 2018Review
- A versatile modular bioreactor platform for Tissue Engineering.Biotechnology journal · 2017Article
- Sinusoidal communication in liver fibrosis and regeneration.Journal of hepatology · 2016Review
- Biology-inspired microphysiological system approaches to solve the prediction dilemma of substance testing.ALTEX · 2016Review
- Microfluidic Organ/Body-on-a-Chip Devices at the Convergence of Biology and Microengineering.Sensors (Basel, Switzerland) · 2015Review
- Microengineered cell and tissue systems for drug screening and toxicology applications: Evolution ofTechnology · 2015Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We describe a unique, versatile bioreactor consisting of two plates and a modified commercial porous membrane suitable for in vitro analysis of the liver sinusoid. The modular bioreactor allows i) excellent control of the cell seeding process; ii) cell culture under controlled shear stress stimulus, and; iii) individual analysis of each cell type upon completion of the experiment. The advantages of the bioreactor detailed here are derived from the modification of a commercial porous membrane with an elastomeric wall specifically moulded in order to define the cell culture area, to act as a gasket that will fit into the bioreactor, and to provide improved mechanical robustness. The device presented herein has been designed to simulate the in vivo organization of a liver sinusoid and tested by co-culturing endothelial cells (EC) and hepatic stellate cells (HSC). The results show both an optimal morphology of the endothelial cells as well as an improvement in the phenotype of stellate cells, most probably due to paracrine factors released from endothelial cells. This device is proposed as a versatile, easy-to-use co-culture system that can be applied to biomedical research of vascular systems, including the liver.
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Registered trials
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