ArticleJournal of insect science (Online)2014
Selection and validation of reference genes for functional studies in the Calliphoridae family.
Article in Journal of insect science (Online), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 35 citations in OpenAlex.
- Screening optimum reference genes for quantitative real-time polymerase chain reaction analysis in black soldier fly Hermetia illucens under different conditions.Insect science · 2026Article
- Stable internal reference genes for quantitative RT-PCR analyses in Rhipicephalus microplus during embryogenesis.Ticks and tick-borne diseases · 2023Article
- Disruption of the odorant coreceptor Orco impairs foraging and host finding behaviors in the New World screwworm fly.Scientific reports · 2021Article
- Physiological and molecular correlates of the screwworm fly attraction to wound and animal odors.Scientific reports · 2020Article
- Sexual-biased gene expression of olfactory-related genes in the antennae of Conogethes pinicolalis (Lepidoptera: Crambidae).BMC genomics · 2020Article
- Evaluation of Candidate Reference Genes for Quantitative Gene Expression Analysis in Spodoptera exigu a after Long-time Exposure to Cadmium.Scientific reports · 2017Article
- Housekeeping in Tephritid insects: the best gene choice for expression analyses in the medfly and the olive fly.Scientific reports · 2017Article
- Evaluation of reference genes at different developmental stages for quantitative real-time PCR in Aedes aegypti.Scientific reports · 2017Article
- DNA-barcoding of forensically important blow flies (Diptera: Calliphoridae) in the Caribbean Region.PeerJ · 2017Article
- Article
- Reference genes for accessing differential expression among developmental stages and analysis of differential expression of OBP genes in Anastrepha obliqua.Scientific reports · 2016Article
- Article
- Reference gene stability of a synanthropic fly, Chrysomya megacephala.Parasites & vectors · 2015Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The genera Cochliomyia and Chrysomya contain both obligate and saprophagous flies, which allows the comparison of different feeding habits between closely related species. Among the different strategies for comparing these habits is the use of qPCR to investigate the expression levels of candidate genes involved in feeding behavior. To ensure an accurate measure of the levels of gene expression, it is necessary to normalize the amount of the target gene with the amount of a reference gene having a stable expression across the compared species. Since there is no universal gene that can be used as a reference in functional studies, candidate genes for qPCR data normalization were selected and validated in three Calliphoridae (Diptera) species, Cochliomyia hominivorax Coquerel, Cochliomyia macellaria Fabricius, and Chrysomya albiceps Wiedemann . The expression stability of six genes ( Actin, Gapdh, Rp49, Rps17, α -tubulin, and GstD1) was evaluated among species within the same life stage and between life stages within each species. The expression levels of Actin, Gapdh, and Rp49 were the most stable among the selected genes. These genes can be used as reliable reference genes for functional studies in Calliphoridae using similar experimental settings.
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