ArticlePloS one2014
Isolation of specific neurons from C. elegans larvae for gene expression profiling.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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Who cites it
60 citing papers in PubMed.
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- Single-type neuron transcriptomics uncovers cell-type-specific regulators of host defense inbioRxiv : the preprint server for biology · 2026Article
- Three muscle-specific DAF-16/FOXO transcriptional targets activated by reduced insulin/IGF-1 signaling.G3 (Bethesda, Md.) · 2026Article
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- A transcriptomic resource for glial GABA-associated ASH neuronal aging and candidate pathways.Frontiers in aging neuroscience · 2026Article
- Intrinsic and non-cell autonomous roles for a neurodevelopmental syndrome-linked transcription factor.bioRxiv : the preprint server for biology · 2025Article
- A gene expression atlas of a juvenile nervous system.bioRxiv : the preprint server for biology · 2025Article
- Decoding sexual dimorphism of the sex-shared nervous system at single-neuron resolution.Science advances · 2025Article
- Alternative splicing across the C. elegans nervous system.Nature communications · 2025Article
- Detecting gene expression in Caenorhabditis elegans.Genetics · 2025Review
- Single-tissue proteomics inProceedings of the National Academy of Sciences of the United States of America · 2024Article
- MAB-5/Hox regulates the Q neuroblast transcriptome, including cwn-1/Wnt, to mediate posterior migration in Caenorhabditis elegans.Genetics · 2024Article
- A molecular atlas of adult C. elegans motor neurons reveals ancient diversity delineated by conserved transcription factor codes.Cell reports · 2024Article
- MAB-5/Hox regulates the Q neuroblast transcriptome, includingbioRxiv : the preprint server for biology · 2023Article
- A molecular atlas of adultbioRxiv : the preprint server for biology · 2023Article
- Spatial single-cell sequencing of meiosis I arrested oocytes indicates acquisition of maternal transcripts from the soma.Cell reports · 2023Article
- Whole-body gene expression atlas of an adult metazoan.Science advances · 2023Article
- The Caenorhabditis elegans anchor cell transcriptome: ribosome biogenesis drives cell invasion through basement membrane.Development (Cambridge, England) · 2023Article
- Femtosecond laser microdissection for isolation of regenerating C. elegans neurons for single-cell RNA sequencing.Nature methods · 2023Article
- Uncovering novel regulators of memory using C. elegans genetic and genomic analysis.Biochemical Society transactions · 2023Review
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8 authors.
Funding
Abstract
backgroundThe simple and well-described structure of the C. elegans nervous system offers an unprecedented opportunity to identify the genetic programs that define the connectivity and function of individual neurons and their circuits. A correspondingly precise gene expression map of C. elegans neurons would facilitate the application of genetic methods toward this goal. Here we describe a powerful new approach, SeqCeL (RNA-Seq of C. elegans cells) for producing gene expression profiles of specific larval C. elegans neurons. METHODS AND
resultsWe have exploited available GFP reporter lines for FACS isolation of specific larval C. elegans neurons for RNA-Seq analysis. Our analysis showed that diverse classes of neurons are accessible to this approach. To demonstrate the applicability of this strategy to rare neuron types, we generated RNA-Seq profiles of the NSM serotonergic neurons that occur as a single bilateral pair of cells in the C. elegans pharynx. These data detected >1,000 NSM enriched transcripts, including the majority of previously known NSM-expressed genes. SIGNIFICANCE: This work offers a simple and robust protocol for expression profiling studies of post-embryonic C. elegans neurons and thus provides an important new method for identifying candidate genes for key roles in neuron-specific development and function.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.