ArticlePloS one2014
Equipment-free incubation of recombinase polymerase amplification reactions using body heat.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 127 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
127 citing papers in PubMed, 1 synthesis or guideline pooled it.
- An update on non-invasive urine diagnostics for human-infecting parasitic helminths: what more could be done and how?Parasitology · 2020Pooled it
- Article
- Point-of-Care Radiation Biodosimetry via a Body-Heated Reverse Transcription-Recombinase Polymerase Amplification Coupled to a Paper-Based Vertical Flow Immunoassay.Analytical chemistry · 2026Article
- Thermostable Bioluminescent Intercalating Dyes for Real-Time, Integrated Nucleic Acid Amplification and Detection.Angewandte Chemie (International ed. in English) · 2026Article
- Establishment of methods for visual and rapid detection of piscine lactococcosis based on isothermal recombinase polymerase amplification.BMC veterinary research · 2026Article
- Establishment and Validation of a Rapid ERA Detection Method forBiosensors · 2026Article
- Diagnosis of leptospirosis in animals: challenges and perspectives.Journal of clinical microbiology · 2026Review
- From Skin to Sequence: Pan-species Detection and Speciation of Leishmania.The Journal of infectious diseases · 2026Article
- A Handy and Visual Detection Method for Potato Late Blight Utilizing Multi-Copy Specific Sequences and RPA-LFD Technology.Journal of microbiology and biotechnology · 2026Article
- Applications of nanoparticles in plant disease identification and control for sustainable crop production.Discover nano · 2026Review
- The molecular revolution in fungal diagnostics: bridging gaps across clinical, agricultural, and environmental mycology.Mycology · 2026Review
- Development of a field-deployable RPA-CRISPR/Cas12a assay for the detection of Cyclospora cayetanensis in human feces.Parasites & vectors · 2025Article
- Development of a novel, rapid, and accurate recombinase polymerase amplification assay for the identification of the glassy-winged sharpshooter,3 Biotech · 2025Article
- Development of a rapid isothermal-based assay for detection of whitefly MEAM1 and MED biotypes using recombinase polymerase amplification.Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Article
- Rapid visual detection of bovine viral diarrhea virus (BVDV) using recombinase polymerase amplification with SYBR green I.BMC veterinary research · 2025Article
- Advances and Challenges in the Diagnosis of Leishmaniasis.Molecular diagnosis & therapy · 2025Review
- Rapid and equipment-free identification of papaya mealybug Paracoccus marginatus based on RPA-CRISPR/Cas12a.Pest management science · 2025Article
- Article
- Rapid and Accurate Detection ofInsects · 2024Article
- Recombinase-aid amplification combined with lateral flow detection assay for sex identification of the great white pelican (Pelecanus onocrotalus).Scientific reports · 2024Article
67 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The development of isothermal amplification platforms for nucleic acid detection has the potential to increase access to molecular diagnostics in low resource settings; however, simple, low-cost methods for heating samples are required to perform reactions. In this study, we demonstrated that human body heat may be harnessed to incubate recombinase polymerase amplification (RPA) reactions for isothermal amplification of HIV-1 DNA. After measuring the temperature of mock reactions at 4 body locations, the axilla was chosen as the ideal site for comfortable, convenient incubation. Using commonly available materials, 3 methods for securing RPA reactions to the body were characterized. Finally, RPA reactions were incubated using body heat while control RPA reactions were incubated in a heat block. At room temperature, all reactions with 10 copies of HIV-1 DNA and 90% of reactions with 100 copies of HIV-1 DNA tested positive when incubated with body heat. In a cold room with an ambient temperature of 10 degrees Celsius, all reactions containing 10 copies or 100 copies of HIV-1 DNA tested positive when incubated with body heat. These results suggest that human body heat may provide an extremely low-cost solution for incubating RPA reactions in low resource settings.
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Registered trials
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