ArticleTheScientificWorldJournal2014
Continuous drip flow system to develop biofilm of E. faecalis under anaerobic conditions.
Article in TheScientificWorldJournal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed, 12 citations in OpenAlex.
- Use of a Cellulase fromPharmaceutics · 2023Article
- Insights Into the Dynamics and Composition of Biofilm Formed by Environmental Isolate ofFrontiers in microbiology · 2022Article
- Development and Quantitation ofBio-protocol · 2021Article
- Establishment and characterization of silver-resistant Enterococcus faecalis.Folia microbiologica · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeTo evaluate a structurally mature E. faecalis biofilm developed under anaerobic/dynamic conditions in an in vitro system.
methodsAn experimental device was developed using a continuous drip flow system designed to develop biofilm under anaerobic conditions. The inoculum was replaced every 24 hours with a fresh growth medium for up to 10 days to feed the system. Gram staining was done every 24 hours to control the microorganism purity. Biofilms developed under the system were evaluated under the scanning electron microscope (SEM).
resultsSEM micrographs demonstrated mushroom-shaped structures, corresponding to a mature E. faecalis biofilm. In the mature biofilm bacterial cells are totally encased in a polymeric extracellular matrix.
conclusionsThe proposed in vitro system model provides an additional useful tool to study the biofilm concept in endodontic microbiology, allowing for a better understanding of persistent root canal infections.
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Registered trials
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