ArticleBioResearch open access2014
Morphological analysis of human induced pluripotent stem cells during induced differentiation and reverse programming.
Article in BioResearch open access, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed.
- Decellularized testicular bio-scaffolds driveFrontiers in bioengineering and biotechnology · 2026Article
- Reporter Alleles in hiPSCs: Visual Cues on Development and Disease.International journal of molecular sciences · 2024Review
- Review
- Generation of Artificial Blastoids Combining miR-200-Mediated Reprogramming and Mechanical Cues.Cells · 2024Article
- Mesenchymal and induced pluripotent stem cell-based therapeutics: a comparison.Applied microbiology and biotechnology · 2023Review
- Feasibility and application of polygenic score analysis to the morphology of human-induced pluripotent stem cells.Molecular genetics and genomics : MGG · 2022Article
- Human induced pluripotent stem cells display a similar mutation burden as embryonic pluripotent cellsiScience · 2022Article
- Miniaturized droplet microarray platform enables maintenance of human induced pluripotent stem cell pluripotency.Materials today. Bio · 2021Article
- Global transcriptome profile of the developmental principles of in vitro iPSC-to-motor neuron differentiation.BMC molecular and cell biology · 2021Article
- Generation of Trophoblast-Like Cells From Hypomethylated Porcine Adult Dermal Fibroblasts.Frontiers in veterinary science · 2021Article
- Acceleration of somatic cell reprogramming into the induced pluripotent stem cell using a mycosporine-like amino acid, Porphyra 334.Scientific reports · 2020Article
- Gain-of-Function Mutant p53: All the Roads Lead to Tumorigenesis.International journal of molecular sciences · 2019Review
- Directing differentiation of human induced pluripotent stem cells toward androgen-producing Leydig cells rather than adrenal cells.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- New tools for cell reprogramming and conversion: Possible applications to livestock.Animal reproduction · 2019Article
- Use of a PTFE Micro-Bioreactor to Promote 3D Cell Rearrangement and Maintain High Plasticity in Epigenetically Erased Fibroblasts.Stem cell reviews and reports · 2019Article
- Media composition modulates human embryonic stem cell morphology and may influence preferential lineage differentiation potential.PloS one · 2019Article
- Contractile Work Contributes to Maturation of Energy Metabolism in hiPSC-Derived Cardiomyocytes.Stem cell reports · 2018Article
- Method for evaluation of human induced pluripotent stem cell quality using image analysis based on the biological morphology of cells.Journal of medical imaging (Bellingham, Wash.) · 2017Article
- Quantitative volumetric Raman imaging of three dimensional cell cultures.Nature communications · 2017Article
- High-throughput physical phenotyping of cell differentiation.Microsystems & nanoengineering · 2017Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The fine analysis of cell components during the generation of pluripotent cells and their comparison to bone fide human embryonic stem cells (hESCs) are valuable tools to understand their biological behavior. In this report, human mesenchymal cells (hMSCs) generated from the human ES cell line H9, were reprogrammed back to induced pluripotent state using Oct-4, Sox2, Nanog, and Lin28 transgenes. Human induced pluripotent stem cells (hIPSCs) were analyzed using electron microscopy and compared with regard to the original hESCs and the hMSCs from which they were derived. This analysis shows that hIPSCs and the original hESCs are morphologically undistinguishable but differ from the hMSCs with respect to the presence of several morphological features of undifferentiated cells at both the cytoplasmic (ribosomes, lipid droplets, glycogen, scarce reticulum) and nuclear levels (features of nuclear plasticity, presence of euchromatin, reticulated nucleoli). We show that hIPSC colonies generated this way presented epithelial aspects with specialized junctions highlighting morphological criteria of the mesenchymal-epithelial transition in cells engaged in a successful reprogramming process. Electron microscopic analysis revealed also specific morphological aspects of partially reprogrammed cells. These results highlight the valuable use of electron microscopy for a better knowledge of the morphological aspects of IPSC and cellular reprogramming.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.