ArticleJournal of experimental botany2015
A mechanism of growth inhibition by abscisic acid in germinating seeds of Arabidopsis thaliana based on inhibition of plasma membrane H+-ATPase and decreased cytosolic pH, K+, and anions.
Article in Journal of experimental botany, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 101 citations in OpenAlex.
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- To grow or not to grow: the enigma of plant root growth dynamism.Plant molecular biology · 2025Review
- Identification and evaluation of low-pH-tolerant Cheongcheong/Nagdong-double haploid rice lines via QTL analysis.BMC plant biology · 2025Article
- SnRK2s: Kinases or Substrates?Plants (Basel, Switzerland) · 2025Review
- Sleeping but not defenceless: seed dormancy and protection.Journal of experimental botany · 2024Review
- Ion Changes and Signaling under Salt Stress in Wheat and Other Important Crops.Plants (Basel, Switzerland) · 2023Review
- A Travel through Landscapes of Seed Dormancy.Plants (Basel, Switzerland) · 2023Review
- Transcription factor TERF1 promotes seed germination through HEXOKINASE 1 (HXK1)-mediated signaling pathway.Journal of plant research · 2023Article
- Transmembrane potential, an indicator in situ reporting cellular senescence and stress response in plant tissues.Plant methods · 2023Article
- The zinc finger protein 3 ofFrontiers in plant science · 2023Article
- FERONIA Receptor Kinase Integrates with Hormone Signaling to Regulate Plant Growth, Development, and Responses to Environmental Stimuli.International journal of molecular sciences · 2022Review
- The Seed and the Metabolism Regulation.Biology · 2022Review
- The root apoplastic pH as an integrator of plant signaling.Frontiers in plant science · 2022Review
- Strigolactones affect phosphorus acquisition strategies in tomato plants.Plant, cell & environment · 2021Article
- Low ABA concentration promotes root growth and hydrotropism through relief of ABA INSENSITIVE 1-mediated inhibition of plasma membrane HScience advances · 2021Article
- Characterization of near-isogenic lines confirmed QTL and revealed candidate genes for plant height and yield-related traits in common wheat.Molecular breeding : new strategies in plant improvement · 2021Article
- The Role of ABA in Plant Immunity is Mediated through the PYR1 Receptor.International journal of molecular sciences · 2020Article
- AnInternational journal of molecular sciences · 2020Article
Corrections and comments
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Authors and funding
12 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The stress hormone abscisic acid (ABA) induces expression of defence genes in many organs, modulates ion homeostasis and metabolism in guard cells, and inhibits germination and seedling growth. Concerning the latter effect, several mutants of Arabidopsis thaliana with improved capability for H(+) efflux (wat1-1D, overexpression of AKT1 and ost2-1D) are less sensitive to inhibition by ABA than the wild type. This suggested that ABA could inhibit H(+) efflux (H(+)-ATPase) and induce cytosolic acidification as a mechanism of growth inhibition. Measurements to test this hypothesis could not be done in germinating seeds and we used roots as the most convenient system. ABA inhibited the root plasma-membrane H(+)-ATPase measured in vitro (ATP hydrolysis by isolated vesicles) and in vivo (H(+) efflux from seedling roots). This inhibition involved the core ABA signalling elements: PYR/PYL/RCAR ABA receptors, ABA-inhibited protein phosphatases (HAB1), and ABA-activated protein kinases (SnRK2.2 and SnRK2.3). Electrophysiological measurements in root epidermal cells indicated that ABA, acting through the PYR/PYL/RCAR receptors, induced membrane hyperpolarization (due to K(+) efflux through the GORK channel) and cytosolic acidification. This acidification was not observed in the wat1-1D mutant. The mechanism of inhibition of the H(+)-ATPase by ABA and its effects on cytosolic pH and membrane potential in roots were different from those in guard cells. ABA did not affect the in vivo phosphorylation level of the known activating site (penultimate threonine) of H(+)-ATPase in roots, and SnRK2.2 phosphorylated in vitro the C-terminal regulatory domain of H(+)-ATPase while the guard-cell kinase SnRK2.6/OST1 did not.
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