ArticleDevelopment (Cambridge, England)2014
Wnt/β-catenin and FGF signalling direct the specification and maintenance of a neuromesodermal axial progenitor in ensembles of mouse embryonic stem cells.
Article in Development (Cambridge, England), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 99 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
99 citing papers in PubMed.
- Distinct regulation of Wnt signaling and EPZ5676 governs human UiPSM reprogramming via the CDX2/T/TBX6 core axis.Cell regeneration (London, England) · 2026Article
- Lineage tracing in gastruloids to understand determinants of early fate decisions.Current opinion in genetics & development · 2026Review
- Sodium Butyrate-Assisted Induction of Posterior Pre-Neural Progenitors from Pluripotent Stem Cells.International journal of molecular sciences · 2026Article
- Investigation of embryogenesis in a dish: the development and application of gastruloid models from pluripotent stem cells.Cell & bioscience · 2026Review
- From genes to germ layers: virtual twins of gastruloids.NPJ systems biology and applications · 2026Review
- Organizers in a dish: Modeling human CNS morphogenesis.Developmental cell · 2026Review
- Wnt/β-catenin signalling modulates the timing of cell fate decision making in the early mouse embryo.PloS one · 2026Article
- Optimization of Gastruloid Pre-culture Conditions for Reproducible Germ Layer Composition.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Impact of TGF-β and ERK1/2 inhibition on preimplantation embryo development and quality.Iranian journal of basic medical sciences · 2026Article
- Posterior enhancer (p-Enh) maintains early neuromesodermal progenitors bi-potency during gastrulation.Cell regeneration (London, England) · 2025Article
- N2B27 media formulations influence gastruloid development.Development (Cambridge, England) · 2025Article
- A New Insight in Cellular and Molecular Signaling Regulation for Neural Differentiation Program.Molecular neurobiology · 2025Review
- The NuRD component CHD3 promotes BMP signalling during cranial neural crest cell specification.EMBO reports · 2025Article
- Fine-tuning mechanical constraints reveals uncoupled patterning and gene expression programs in murine gastruloids.Development (Cambridge, England) · 2025Article
- Size-dependent temporal decoupling of morphogenesis and transcriptional programs in pseudoembryos.Science advances · 2025Article
- Progress in understanding the vertebrate segmentation clock.Nature reviews. Genetics · 2025Review
- Signaling Centers Drive Brachyury Dynamics and Lineage Commitment in mESC Aggregates.FASEB bioAdvances · 2025Article
- Dual genetic tracing demonstrates the heterogeneous differentiation and function of neuromesodermal progenitors in vivo.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- The potent neuroepithelium-promoting activity of Otx2 during gastrulation, as demonstrated by its exogenous epiblast-wide expression in chicken embryos.Frontiers in cell and developmental biology · 2025Article
- Stem cell culture conditions affect in vitro differentiation potential and mouse gastruloid formation.PloS one · 2025Article
39 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
The development of the central nervous system is known to result from two sequential events. First, an inductive event of the mesoderm on the overlying ectoderm that generates a neural plate that, after rolling into a neural tube, acts as the main source of neural progenitors. Second, the axial regionalization of the neural plate that will result in the specification of neurons with different anteroposterior identities. Although this description of the process applies with ease to amphibians and fish, it is more difficult to confirm in amniote embryos. Here, a specialized population of cells emerges at the end of gastrulation that, under the influence of Wnt and FGF signalling, expands and generates the spinal cord and the paraxial mesoderm. This population is known as the long-term neuromesodermal precursor (NMp). Here, we show that controlled increases of Wnt/β-catenin and FGF signalling during adherent culture differentiation of mouse embryonic stem cells (mESCs) generates a population with many of the properties of the NMp. A single-cell analysis of gene expression within this population reveals signatures that are characteristic of stem cell populations. Furthermore, when this activation is triggered in three-dimensional aggregates of mESCs, the population self-organizes macroscopically and undergoes growth and axial elongation that mimics some of the features of the embryonic spinal cord and paraxial mesoderm. We use both adherent and three-dimensional cultures of mESCs to probe the establishment and maintenance of NMps and their differentiation.
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