ArticleJournal of proteome research2015
Complete and ubiquitinated proteome of the Legionella-containing vacuole within human macrophages.
Article in Journal of proteome research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 35 citations in OpenAlex.
- A survey of ADP-ribosyltransferase families in the pathogenic Legionella.BMC genomics · 2025Article
- Article
- Potential probiotic approaches to control Legionella in engineered aquatic ecosystems.FEMS microbiology ecology · 2022Review
- Proteome analysis ofFrontiers in cellular and infection microbiology · 2022Article
- Protein and DNA Biosynthesis Demonstrated in Host Cell-Free Phagosomes Containing Anaplasma phagocytophilum or Ehrlichia chaffeensis in Axenic Media.Infection and immunity · 2021Article
- Modification of the host ubiquitome by bacterial enzymes.Microbiological research · 2020Review
- A MicroRNA Network ControlsmBio · 2020Article
- Nutrition and Bipartite Metabolism of Intracellular Pathogens.Trends in microbiology · 2019Review
- Human Intestinal Enteroids as a Model System ofInfection and immunity · 2019Article
- The recycling endosome and bacterial pathogens.Cellular microbiology · 2018Review
- Divergent evolution of Di-lysine ER retention vs. farnesylation motif-mediated anchoring of the AnkB virulence effector to the Legionella-containing vacuolar membrane.Scientific reports · 2017Article
- Type II Secretion Substrates ofmBio · 2017Article
- Comparative Proteomics of Purified Pathogen Vacuoles Correlates Intracellular Replication ofMolecular & cellular proteomics : MCP · 2017Article
- Structural Mimicry by a Bacterial F Box Effector Hijacks the Host Ubiquitin-Proteasome System.Structure (London, England : 1993) · 2017Article
- The Toolbox for Uncovering the Functions ofFrontiers in cellular and infection microbiology · 2017Review
- Host FIH-Mediated Asparaginyl Hydroxylation of TranslocatedFrontiers in cellular and infection microbiology · 2017Article
- Isolation ofFrontiers in cellular and infection microbiology · 2017Article
- Type II Secretion Is Necessary for Optimal Association of the Legionella-Containing Vacuole with Macrophage Rab1B but Enhances Intracellular Replication Mainly by Rab1B-Independent Mechanisms.Infection and immunity · 2016Article
- Selective requirement of the shikimate pathway of Legionella pneumophila for intravacuolar growth within human macrophages but not within Acanthamoeba.Infection and immunity · 2015Article
- Host Delivery of Favorite Meals for Intracellular Pathogens.PLoS pathogens · 2015Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Within protozoa or human macrophages Legionella pneumophila evades the endosomal pathway and replicates within an ER-derived vacuole termed the Legionella-containing vacuole (LCV). The LCV membrane-localized AnkB effector of L. pneumophila is an F-box protein that mediates decoration of the LCV with lysine(48)-linked polyubiquitinated proteins, which is essential for intravacuolar replication. Using high-throughput LC-MS analysis, we have identified the total and ubiquitinated host-derived proteome of LCVs purified from human U937 macrophages. The LCVs harboring the AA100/130b WT strain contain 1193 proteins including 24 ubiquitinated proteins, while the ankB mutant LCVs contain 1546 proteins with 29 ubiquitinated proteins. Pathway analyses reveal the enrichment of proteins involved in signaling, protein transport, phosphatidylinositol, and carbohydrate metabolism on both WT and ankB mutant LCVs. The ankB mutant LCVs are preferentially enriched for proteins involved in transcription/translation and immune responses. Ubiquitinated proteins on the WT strain LCVs are enriched for immune response, signaling, regulation, intracellular trafficking, and amino acid transport pathways, while ubiquitinated proteins on the ankB mutant LCVs are enriched for vesicle trafficking, signaling, and ubiquitination pathways. The complete and ubiquitinated LCV proteome within human macrophages illustrates complex and dynamic biogenesis of the LCV and provides a rich resource for future studies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.