ArticleJournal of biomedical materials research. Part A2015
Investigating thiol-modification on hyaluronan via carbodiimide chemistry using response surface methodology.
Article in Journal of biomedical materials research. Part A, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed.
- Crosslinking-Dependent Design of Hyaluronic Acid Matrices for Enhanced Bioadhesion and Cellular Response.Pharmaceutics · 2026Article
- Mussel-Inspired Catechol-Functionalized Redox-Active Polypeptides for Energy Applications.Biomacromolecules · 2026Article
- Thiol-Functionalized Succinoglycan via Cysteine Grafting: Enhanced Rheological Properties and Antioxidant Activity.Polymers · 2026Article
- Functional Properties of Chitosan Conjugated with Oleic Acid and Caffeic Acid and Its Application in Oil-in-Water Emulsions.Molecules (Basel, Switzerland) · 2026Article
- Marine Biomaterials: Hyaluronan.Marine drugs · 2023Review
- A LC-QTOF Method for the Determination of PEGDE Residues in Dermal Fillers.Gels (Basel, Switzerland) · 2023Article
- Optimizing the composition of gelatin methacryloyl and hyaluronic acid methacryloyl hydrogels to maximize mechanical and transport properties using response surface methodology.Journal of biomedical materials research. Part B, Applied biomaterials · 2023Article
- Article
- Formulation of Magneto-Responsive Hydrogels from Dually Cross-Linked Polysaccharides: Synthesis, Tuning and Evaluation of Rheological Properties.International journal of molecular sciences · 2022Article
- Chemo-photothermal immunotherapy for eradication of orthotopic tumors and inhibition of metastasis by intratumoral injection of polydopamine versatile hydrogels.Acta pharmaceutica Sinica. B · 2022Article
- Physico-Chemical Characterization and In Vitro Biological Evaluation of a Bionic Hydrogel Based on Hyaluronic Acid and l-Lysine for Medical Applications.Pharmaceutics · 2021Article
- Hyaluronan-Conjugated Carbon Quantum Dots for Bioimaging Use.ACS applied materials & interfaces · 2021Article
- Review
- Long-term expansion, genomic stability and in vivo safety of adult human pancreas organoids.BMC developmental biology · 2020Article
- Hyaluronate coating enhances the delivery and biocompatibility of gold nanoparticles.Carbohydrate polymers · 2018Article
- Reduction-sensitive CD44 receptor-targeted hyaluronic acid derivative micelles for doxorubicin delivery.International journal of nanomedicine · 2018Article
- Synthesis and Characterization of Injectable Sulfonate-Containing Hydrogels.Biomacromolecules · 2016Article
- Investigating triazine-based modification of hyaluronan using statistical designs.Carbohydrate polymers · 2015Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Hyaluronan (HA) is a naturally occurring glycosaminoglycan widely researched for its use as a biomaterial in tissue engineering, drug delivery, angiogenesis, and ophthalmic surgeries. The mechanical properties of this biomaterial can be altered to a required extent by chemically modifying the pendant reactive groups. However, derivatizing these polymers to a predetermined extent has been the Achilles heel for this process. In this study, we have investigated the factors controlling the derivatization of the carboxyl moieties of HA with amine containing thiol, cystamine dihydrochloride (Cys), via carbodiimide crosslinking chemistry. We used fractional factorial design to screen and identify the significant factor(s) affecting the reaction, and response surface methodology (RSM) to develop a model equation for predicting the degree of thiolation of HA. Also, we analyzed the reaction mechanism for potential side reactions. We observed that N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC) (mole ratio with repeat unit of HA) is the significant factor controlling the degree of amidation. The quadratic equations developed from RSM predict the formulation for a desired degree of amidation of HA and percentage of potential side product. Hence, derivatizing HA to a predetermined extent with minimal side product can be achieved using the statistical design of experiments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.