ArticleMolecular and cellular biology2015
A variably occupied CTCF binding site in the ultrabithorax gene in the Drosophila bithorax complex.
Article in Molecular and cellular biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 23 citations in OpenAlex.
- 3D chromatin structures precede genome activation in Drosophila embryogenesis.Cell genomics · 2025Article
- Enhancer-promoter interactions become more instructive in the transition from cell-fate specification to tissue differentiation.Nature genetics · 2024Article
- Characterisation of the role and regulation ofFrontiers in cell and developmental biology · 2023Article
- The Variable CTCF Site from Drosophila melanogaster Ubx Gene is Redundant and Has no Insulator Activity.Doklady. Biochemistry and biophysics · 2022Article
- Systematic screening of CTCF binding partners identifies that BHLHE40 regulates CTCF genome-wide distribution and long-range chromatin interactions.Nucleic acids research · 2020Article
- TET-Catalyzed 5-Carboxylcytosine Promotes CTCF Binding to Suboptimal Sequences Genome-wide.iScience · 2019Article
- Complete reconstitution of bypass and blocking functions in a minimal artificialProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Boundaries mediate long-distance interactions between enhancers and promoters in the Drosophila Bithorax complex.PLoS genetics · 2018Article
- Architectural protein Pita cooperates with dCTCF in organization of functional boundaries in Bithorax complex.Development (Cambridge, England) · 2017Article
- Functional assessment of CTCF sites at cytokine-sensing mammary enhancers using CRISPR/Cas9 gene editing in mice.Nucleic acids research · 2017Article
- Boundaries of loop domains (insulators): Determinants of chromosome form and function in multicellular eukaryotes.BioEssays : news and reviews in molecular, cellular and developmental biology · 2017Review
- Regulation of the BMP Signaling-Responsive Transcriptional Network in the Drosophila Embryo.PLoS genetics · 2016Article
- Maintenance of a Drosophila melanogaster Population Cage.Journal of visualized experiments : JoVE · 2016Article
- The boundary paradox in the Bithorax complex.Mechanisms of development · 2015Article
- The ubiquitous transcription factor CTCF promotes lineage-specific epigenomic remodeling and establishment of transcriptional networks driving cell differentiation.Nucleus (Austin, Tex.) · 2015Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Although the majority of genomic binding sites for the insulator protein CCCTC-binding factor (CTCF) are constitutively occupied, a subset show variable occupancy. Such variable sites provide an opportunity to assess context-specific CTCF functions in gene regulation. Here, we have identified a variably occupied CTCF site in the Drosophila Ultrabithorax (Ubx) gene. This site is occupied in tissues where Ubx is active (third thoracic leg imaginal disc) but is not bound in tissues where the Ubx gene is repressed (first thoracic leg imaginal disc). Using chromatin conformation capture, we show that this site preferentially interacts with the Ubx promoter region in the active state. The site lies close to Ubx enhancer elements and is also close to the locations of several gypsy transposon insertions that disrupt Ubx expression, leading to the bx mutant phenotype. gypsy insertions carry the Su(Hw)-dependent gypsy insulator and were found to affect both CTCF binding at the variable site and the chromatin topology. This suggests that insertion of the gypsy insulator in this region interferes with CTCF function and supports a model for the normal function of the variable CTCF site as a chromatin loop facilitator, promoting interaction between Ubx enhancers and the Ubx transcription start site.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.