ArticlePhilosophical transactions. Series A, Mathematical, physical, and engineering sciences2014
Potential and limits of Raman spectroscopy for carotenoid detection in microorganisms: implications for astrobiology.
Article in Philosophical transactions. Series A, Mathematical, physical, and engineering sciences, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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24 citing papers in PubMed, 99 citations in OpenAlex.
- RamanOmics decodes the spatial vibrational-molecular architecture of senescence in aging and repair.Nature aging · 2026Article
- Machine learning-assisted single-cell Raman imaging for rapid, sensitive detection and intracellular mapping of carotenoids in plant cell cultures.Plant cell reports · 2026Article
- Calcite precipitation by the nitrogen-fixing cyanobacterium Anabaena sp. ATCC 33047.Communications biology · 2025Article
- Single-cell pigment analysis of phototrophic and phyllosphere bacteria using simultaneous detection of Raman and autofluorescence spectra.Applied and environmental microbiology · 2025Article
- Multimodal non-invasive probing of stress-induced carotenogenesis in the cells of microalga Bracteacoccus aggregatus.Protoplasma · 2024Article
- Uncovering the Role of Autochthonous Deteriogenic Biofilm Community: Rožanec Mithraeum Monument (Slovenia).Microbial ecology · 2024Article
- Association of Acidotolerant Cyanobacteria to Microbial Mats below pH 1 in Acidic Mineral Precipitates in Río Tinto River in Spain.Microorganisms · 2024Article
- Global biochemical profiling of fast-growing Antarctic bacteria isolated from meltwater ponds by high-throughput FTIR spectroscopy.PloS one · 2024Article
- Isomerization of carotenoids in photosynthesis and metabolic adaptation.Biophysical reviews · 2023Review
- Vibrational Spectroscopy as a Tool for Bioanalytical and Biomonitoring Studies.International journal of molecular sciences · 2023Review
- Gypsum endolithic phototrophs under moderate climate (Southern Sicily): their diversity and pigment composition.Frontiers in microbiology · 2023Article
- Differentiation of advanced generation mutant wheat lines: Conventional techniques versus Raman spectroscopy.Frontiers in plant science · 2023Article
- Quantification of salt stress in wheat leaves by Raman spectroscopy and machine learning.Scientific reports · 2022Article
- Article
- Review
- Structure elucidation of the novel carotenoid gemmatoxanthin from the photosynthetic complex of Gemmatimonas phototrophica AP64.Scientific reports · 2021Article
- Photo-Disassembly of Membrane Microdomains Revives Conventional Antibiotics against MRSA.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2020Article
- Non-Invasive Characterization of Single-, Double- and Triple-Viral Diseases of Wheat With a Hand-Held Raman Spectrometer.Frontiers in plant science · 2020Article
- Rapid and Noninvasive Typing and Assessment of Nutrient Content of Maize Kernels Using a Handheld Raman Spectrometer.ACS omega · 2019Article
- Photosensitivity responses ofRSC advances · 2019Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this paper, it is demonstrated how Raman spectroscopy can be used to detect different carotenoids as possible biomarkers in various groups of microorganisms. The question which arose from previous studies concerns the level of unambiguity of discriminating carotenoids using common Raman microspectrometers. A series of laboratory-grown microorganisms of different taxonomic affiliation was investigated, such as halophilic heterotrophic bacteria, cyanobacteria, the anoxygenic phototrophs, the non-halophilic heterotrophs as well as eukaryotes (Ochrophyta, Rhodophyta and Chlorophyta). The data presented show that Raman spectroscopy is a suitable tool to assess the presence of carotenoids of these organisms in cultures. Comparison is made with the high-performance liquid chromatography approach of analysing pigments in extracts. Direct measurements on cultures provide fast and reliable identification of the pigments. Some of the carotenoids studied are proposed as tracers for halophiles, in contrast with others which can be considered as biomarkers of other genera. The limits of application of Raman spectroscopy are discussed for a few cases where the current Raman spectroscopic approach does not allow discriminating structurally very similar carotenoids. The database reported can be used for applications in geobiology and exobiology for the detection of pigment signals in natural settings.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.