ArticleThe Journal of biological chemistry2014
Functional analysis of insect molting fluid proteins on the protection and regulation of ecdysis.
Article in The Journal of biological chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 106 citations in OpenAlex.
- N-Acetylcysteine Attacks Phenoloxidase to Inhibit Insect Melanization and Boost Fungal Virulence.Journal of fungi (Basel, Switzerland) · 2026Article
- Molting in Pancrustacea Is Characterized by Both Deeply Conserved and Recently Evolved Gene Modules.Genome biology and evolution · 2026Article
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- TheProceedings of the National Academy of Sciences of the United States of America · 2025Article
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- Comparative transcriptome analysis to unveil genes affecting the host cuticle destruction in Metarhizium rileyi.Current genetics · 2023Article
- Gene expression in Verson's glands of the fall armyworm suggests their role in molting and immunity.Frontiers in insect science · 2023Article
- Bacterial ectosymbionts in cuticular organs chemically protect a beetle during molting stages.The ISME journal · 2022Article
- Proteomic analysis of pharate pupal molting fluid from the tobacco hornworm, Manduca sexta.Insect biochemistry and molecular biology · 2022Article
- Phenotypic analyses, protein localization, and bacteriostatic activity of Drosophila melanogaster transferrin-1.Insect biochemistry and molecular biology · 2022Article
- cDNA Cloning and Partial Characterization of theAntioxidants (Basel, Switzerland) · 2021Article
- An apocrine mechanism delivers a fully immunocompetent exocrine secretion.Scientific reports · 2021Article
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- Ablation of Selenbp1 Alters Lipid Metabolism via the Pparα Pathway in Mouse Kidney.International journal of molecular sciences · 2021Article
- Physiological characterization of chitin synthase A responsible for the biosynthesis of cuticle chitin in Culex pipiens pallens (Diptera: Culicidae).Parasites & vectors · 2021Article
- Article
- Phylogenetic and sequence analyses of insect transferrins suggest that only transferrin 1 has a role in iron homeostasis.Insect science · 2021Article
- Transcriptomic response of Anopheles gambiae sensu stricto mosquito larvae to Curry tree (Murraya koenigii) phytochemicals.Parasites & vectors · 2021Article
- Involvement of Epidermis Cell Proliferation in Defense AgainstFrontiers in immunology · 2021Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Molting fluid accumulates between the old and new cuticles during periodical ecdysis in Ecdysozoa. Natural defects in insect ecdysis are frequently associated with melanization (an immunity response) occurring primarily in molting fluids, suggesting that molting fluid may impact immunity as well as affect ecdysis. To address this hypothesis, proteomic analysis of molting fluids from Bombyx mori during three different types of ecdysis was performed. Many proteins were newly identified, including immunity-related proteins, in each molting fluid. Molting fluids inhibited the growth of bacteria in vitro. The entomopathogenic fungi Beauveria bassiana, which can escape immune responses in feeding larvae, is quickly recognized by larvae during ecdysis, followed by melanization in molting fluid and old cuticle. Fungal conidia germination was delayed, and no hyphae were detected in the hemocoels of pharate instar insects. Molting fluids protect the delicate pharate instar insects with extremely thin cuticles against microorganisms. To explore the function of molting fluids in ecdysis regulation, based on protein similarity, 32 genes were selected for analysis in ecdysis regulation through RNAi in Tribolium castaneum, a model commonly used to study integument development because RNAi is difficult to achieve in B. mori. We identified 24 molting proteins that affected ecdysis after knockdown, with different physiological functions, including old cuticle protein recycling, molting fluid pressure balance, detoxification, and signal detection and transfer of molting fluids. We report that insects secrete molting fluid for protection and regulation of ecdysis, which indicates a way to develop new pesticides through interrupting insect ecdysis in the future.
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