ArticleProceedings of the National Academy of Sciences of the United States of America2014
Single gene insertion drives bioalcohol production by a thermophilic archaeon.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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47 citing papers in PubMed, 103 citations in OpenAlex.
- Iron-Containing Alcohol Dehydrogenase from Hyperthermophiles.Biotech (Basel (Switzerland)) · 2026Review
- Carbon-monoxide-driven bioethanol production operates through a tungsten-dependent catalyst.Nature chemical biology · 2026Article
- Activation-Free Upgrading of Carboxylic Acids to Aldehydes and Alcohols.bioRxiv : the preprint server for biology · 2025Article
- Engineering the hyperthermophilic archaeonApplied and environmental microbiology · 2025Article
- Obligately Tungsten-Dependent Enzymes─Catalytic Mechanisms, Models and Applications.Biochemistry · 2025Review
- Isolation and characterization of novel acetogenic Moorella strains for employment as potential thermophilic biocatalysts.FEMS microbiology ecology · 2024Article
- Extremophiles in a changing world.Extremophiles : life under extreme conditions · 2024Review
- A Versatile Aldehyde: Ferredoxin Oxidoreductase from the Organic Acid ReducingInternational journal of molecular sciences · 2024Article
- Acetic acid, growth rate, and mass transfer govern shifts in CO metabolism of Clostridium autoethanogenum.Applied microbiology and biotechnology · 2023Article
- Manipulating Fermentation Pathways in the Hyperthermophilic ArchaeonApplied and environmental microbiology · 2023Article
- Optimizing Strategies for Bio-Based Ethanol Production Using Genome-Scale Metabolic Modeling of the Hyperthermophilic Archaeon, Pyrococcus furiosus.Applied and environmental microbiology · 2023Article
- A bacterial tungsten-containing aldehyde oxidoreductase forms an enzymatic decorated protein nanowire.Science advances · 2023Article
- Characterization ofBiology · 2022Article
- Tungsten enzymes play a role in detoxifying food and antimicrobial aldehydes in the human gut microbiome.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Alcohol dehydrogenases AdhE and AdhB with broad substrate ranges are important enzymes for organic acid reduction in Thermoanaerobacter sp. strain X514.Biotechnology for biofuels · 2021Article
- Life in hot acid: a genome-based reassessment of the archaeal order Sulfolobales.Environmental microbiology · 2021Article
- Propionate Production from Carbon Monoxide by Synthetic Cocultures of Acetobacterium wieringae and Propionigenic Bacteria.Applied and environmental microbiology · 2021Article
- A novel bifunctional aldehyde/alcohol dehydrogenase catalyzing reduction of acetyl-CoA to ethanol at temperatures up to 95 °C.Scientific reports · 2021Article
- Co-feeding glucose with either gluconate or galacturonate during clostridial fermentations provides metabolic fine-tuning capabilities.Scientific reports · 2021Article
- Engineering the cellulolytic extreme thermophile Caldicellulosiruptor bescii to reduce carboxylic acids to alcohols using plant biomass as the energy source.Journal of industrial microbiology & biotechnology · 2020Article
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bioethanol production is achieved by only two metabolic pathways and only at moderate temperatures. Herein a fundamentally different synthetic pathway for bioalcohol production at 70 °C was constructed by insertion of the gene for bacterial alcohol dehydrogenase (AdhA) into the archaeon Pyrococcus furiosus. The engineered strain converted glucose to ethanol via acetate and acetaldehyde, catalyzed by the host-encoded aldehyde ferredoxin oxidoreductase (AOR) and heterologously expressed AdhA, in an energy-conserving, redox-balanced pathway. Furthermore, the AOR/AdhA pathway also converted exogenously added aliphatic and aromatic carboxylic acids to the corresponding alcohol using glucose, pyruvate, and/or hydrogen as the source of reductant. By heterologous coexpression of a membrane-bound carbon monoxide dehydrogenase, CO was used as a reductant for converting carboxylic acids to alcohols. Redirecting the fermentative metabolism of P. furiosus through strategic insertion of foreign genes creates unprecedented opportunities for thermophilic bioalcohol production. Moreover, the AOR/AdhA pathway is a potentially game-changing strategy for syngas fermentation, especially in combination with carbon chain elongation pathways.
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