ArticleProceedings of the National Academy of Sciences of the United States of America2014
Sexual transfer of the steroid hormone 20E induces the postmating switch in Anopheles gambiae.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 76 papers.
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Who cites it
76 citing papers in PubMed, 135 citations in OpenAlex.
- Article
- Two neuropeptides that promote blood feeding ineLife · 2026Article
- Insect Sterols and Steroids.Advances in experimental medicine and biology · 2026Review
- Age-dependent mating dynamics, body size, and sex ratio in colonisedCurrent research in parasitology & vector-borne diseases · 2026Article
- Insulin-like peptides activate egg formation in the Asian malaria mosquito Anopheles stephensi.Parasites & vectors · 2025Article
- Article
- β2-tubulin regulates the development and migration of eupyrene sperm in Spodoptera frugiperda.Cellular and molecular life sciences : CMLS · 2025Article
- A novel male accessory gland peptide reduces female post-mating receptivity in the brown planthopper.PLoS genetics · 2025Article
- Article
- Defining the genetics of the widely used G3 strain of the mosquito, Anopheles gambiae.Scientific reports · 2025Article
- Warmer temperature accelerates reproductive senescence in mosquitoes.Frontiers in physiology · 2025Article
- Deafness due to loss of a TRPV channel eliminates mating behavior inProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Molecular correlates of swarming behaviour inBiology letters · 2024Article
- Eliminating malaria vectors with precision-guided sterile males.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Juvenile Hormone as a contributing factor in establishing midgut microbiota for fecundity and fitness enhancement in adult female Aedes aegypti.Communications biology · 2024Article
- CRISPR-mediated germline mutagenesis for genetic sterilization of Anopheles gambiae males.Scientific reports · 2024Article
- Identification of genes regulated by 20-Hydroxyecdysone in Macrobrachium nipponense using comparative transcriptomic analysis.BMC genomics · 2024Article
- Current Status of Omics Studies Elucidating the Features of Reproductive Biology in Blood-Feeding Insects.Insects · 2023Review
- Review
- Eliminating Malaria Vectors with Precision Guided Sterile Males.bioRxiv : the preprint server for biology · 2023Article
16 more citing papers are in PubMed but not listed here.
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Authors and funding
9 authors at 3 institutions in 3 countries.
Funding
Abstract
Female insects generally mate multiple times during their lives. A notable exception is the female malaria mosquito Anopheles gambiae, which after sex loses her susceptibility to further copulation. Sex in this species also renders females competent to lay eggs developed after blood feeding. Despite intense research efforts, the identity of the molecular triggers that cause the postmating switch in females, inducing a permanent refractoriness to further mating and triggering egg-laying, remains elusive. Here we show that the male-transferred steroid hormone 20-hydroxyecdysone (20E) is a key regulator of monandry and oviposition in An. gambiae. When sexual transfer of 20E is impaired by partial inactivation of the hormone and inhibition of its biosynthesis in males, oviposition and refractoriness to further mating in the female are strongly reduced. Conversely, mimicking sexual delivery by injecting 20E into virgin females switches them to an artificial mated status, triggering egg-laying and reducing susceptibility to copulation. Sexual transfer of 20E appears to incapacitate females physically from receiving seminal fluids by a second male. Comparative analysis of microarray data from females after mating and after 20E treatment indicates that 20E-regulated molecular pathways likely are implicated in the postmating switch, including cytoskeleton and musculature-associated genes that may render the atrium impenetrable to additional mates. By revealing signals and pathways shaping key processes in the An. gambiae reproductive biology, our data offer new opportunities for the control of natural populations of malaria vectors.
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