ArticleProceedings of the National Academy of Sciences of the United States of America2014
Isotopically nonstationary 13C flux analysis of changes in Arabidopsis thaliana leaf metabolism due to high light acclimation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 114 papers.
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114 citing papers in PubMed.
- Chorismate and isochorismate turnover reveal hidden dynamics of aromatic metabolism in Arabidopsis leaves.The Plant cell · 2026Article
- Essential mitochondrial activity in Physcomitrium patens relies on complementary cytochrome and alternative oxidase pathways.The Plant journal : for cell and molecular biology · 2026Article
- Conserved leaf-root metabolomic network asymmetry underpins divergent drought strategies.The Plant cell · 2026Article
- How Plants May Maintain Protein Homeostasis Under Rising Atmospheric COPlant, cell & environment · 2026Review
- Precursor-Dependent Routing of Aromatic Amino Acids Determines Lignin Structure in Grasses by Sensitivity-Enhanced Solid-State NMR.Journal of the American Chemical Society · 2026Article
- Understanding plant resilience by putting photosynthesis and photorespiration in the metabolic context.Planta · 2026Review
- Metabolic rewiring and biomass redistribution enable optimized mixotrophic growth in Chlamydomonas.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Precursor-Dependent Routing of Aromatic Amino Acids Determines Lignin Structure in Grasses by Sensitivity-Enhanced Solid-State NMR.bioRxiv : the preprint server for biology · 2026Article
- Carbon Dioxide Release During Photosynthesis: Connecting Gas Exchange Behavior With Biochemistry.Plant, cell & environment · 2026Review
- Time-resolved targeted metabolomics shows an abrupt switch from Calvin-Benson-Bassham cycle to tricarboxylic acid cycle when the light is turned off.Photosynthesis research · 2025Article
- Metabolic flux analysis in leaf metabolism quantifies the link between photorespiration and one carbon metabolism.Nature plants · 2025Article
- Non-canonical plant metabolism.Nature plants · 2025Review
- Alternatives to photorespiration: A system-level analysis reveals mechanisms of enhanced plant productivity.Science advances · 2025Article
- The effects of photosynthetic rate on respiration in light, starch/sucrose partitioning, and other metabolic fluxes within photosynthesis.Scientific reports · 2025Article
- Review
- Metabolic flux analysis to increase oil in seeds.Plant physiology · 2025Review
- A Guide to Metabolic Network Modeling for Plant Biology.Plants (Basel, Switzerland) · 2025Review
- Article
- Deciphering sphingolipid biosynthesis dynamics iniScience · 2024Article
- Central transcriptional regulator controls photosynthetic growth and carbon storage in response to high light.Nature communications · 2024Article
54 more citing papers are in PubMed but not listed here.
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4 authors.
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Abstract
Improving plant productivity is an important aim for metabolic engineering. There are few comprehensive methods that quantitatively describe leaf metabolism, although such information would be valuable for increasing photosynthetic capacity, enhancing biomass production, and rerouting carbon flux toward desirable end products. Isotopically nonstationary metabolic flux analysis (INST-MFA) has been previously applied to map carbon fluxes in photoautotrophic bacteria, which involves model-based regression of transient (13)C-labeling patterns of intracellular metabolites. However, experimental and computational difficulties have hindered its application to terrestrial plant systems. We performed in vivo isotopic labeling of Arabidopsis thaliana rosettes with (13)CO2 and estimated fluxes throughout leaf photosynthetic metabolism by INST-MFA. Plants grown at 200 µmol m(-2)s(-1) light were compared with plants acclimated for 9 d at an irradiance of 500 µmol⋅m(-2)⋅s(-1). Approximately 1,400 independent mass isotopomer measurements obtained from analysis of 37 metabolite fragment ions were regressed to estimate 136 total fluxes (54 free fluxes) under each condition. The results provide a comprehensive description of changes in carbon partitioning and overall photosynthetic flux after long-term developmental acclimation of leaves to high light. Despite a doubling in the carboxylation rate, the photorespiratory flux increased from 17 to 28% of net CO2 assimilation with high-light acclimation (Vc/Vo: 3.5:1 vs. 2.3:1, respectively). This study highlights the potential of (13)C INST-MFA to describe emergent flux phenotypes that respond to environmental conditions or plant physiology and cannot be obtained by other complementary approaches.
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