ArticlePlant physiology2015
Alternative splicing-mediated targeting of the Arabidopsis GLUTAMATE RECEPTOR3.5 to mitochondria affects organelle morphology.
Article in Plant physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 78 citations in OpenAlex.
- Amino Acids as Multifunctional Molecules in Plants: From Fundamental Metabolism to Precision Agriculture.Plants (Basel, Switzerland) · 2026Review
- The important role of chloroplasts in plant immunity.Plant communications · 2025Review
- Exploring the regulatory roles of AtGLR3.4 receptors in mitochondrial stress and ROS management in Arabidopsis.Plant cell reports · 2025Article
- Diatoms exhibit dynamic chloroplast calcium signals in response to high light and oxidative stress.Plant physiology · 2024Article
- E3 SUMO ligase SIZ1 splicing variants localize and function according to external conditions.Plant physiology · 2024Article
- Channels of Evolution: Unveiling Evolutionary Patterns in Diatom CaPlants (Basel, Switzerland) · 2024Article
- UMAMIT44 is a key player in glutamate export from Arabidopsis chloroplasts.The Plant cell · 2024Article
- Article
- AnPlant signaling & behavior · 2023Article
- Genome-wide identification of the glutamate receptor-like gene family inPlant signaling & behavior · 2023Article
- Article
- Merging Signaling with Structure: Functions and Mechanisms of Plant Glutamate Receptor Ion Channels.Annual review of plant biology · 2023Review
- Comparative analysis of wild-type and chloroplast MCU-deficient plants reveals multiple consequences of chloroplast calcium handling under drought stress.Frontiers in plant science · 2023Article
- Roles of Glutamate Receptor-Like Channels (GLRs) in Plant Growth and Response to Environmental Stimuli.Plants (Basel, Switzerland) · 2022Review
- MCU proteins dominate in vivo mitochondrial Ca2+ uptake in Arabidopsis roots.The Plant cell · 2022Article
- An Insight into Animal Glutamate Receptors Homolog ofPlants (Basel, Switzerland) · 2022Review
- Upregulation of GLRs expression by light in Arabidopsis leaves.BMC plant biology · 2022Article
- The signatures of organellar calcium.Plant physiology · 2021Review
- Transport, functions, and interaction of calcium and manganese in plant organellar compartments.Plant physiology · 2021Review
- Adaptive Responses ofInternational journal of molecular sciences · 2021Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Since the discovery of 20 genes encoding for putative ionotropic glutamate receptors in the Arabidopsis (Arabidopsis thaliana) genome, there has been considerable interest in uncovering their physiological functions. For many of these receptors, neither their channel formation and/or physiological roles nor their localization within the plant cells is known. Here, we provide, to our knowledge, new information about in vivo protein localization and give insight into the biological roles of the so-far uncharacterized Arabidopsis GLUTAMATE RECEPTOR3.5 (AtGLR3.5), a member of subfamily 3 of plant glutamate receptors. Using the pGREAT vector designed for the expression of fusion proteins in plants, we show that a splicing variant of AtGLR3.5 targets the inner mitochondrial membrane, while the other variant localizes to chloroplasts. Mitochondria of knockout or silenced plants showed a strikingly altered ultrastructure, lack of cristae, and swelling. Furthermore, using a genetically encoded mitochondria-targeted calcium probe, we measured a slightly reduced mitochondrial calcium uptake capacity in the knockout mutant. These observations indicate a functional expression of AtGLR3.5 in this organelle. Furthermore, AtGLR3.5-less mutant plants undergo anticipated senescence. Our data thus represent, to our knowledge, the first evidence of splicing-regulated organellar targeting of a plant ion channel and identify the first cation channel in plant mitochondria from a molecular point of view.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.