ArticlePlant physiology2014
Microtubules contribute to tubule elongation and anchoring of endoplasmic reticulum, resulting in high network complexity in Arabidopsis.
Article in Plant physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 67 citations in OpenAlex.
- An ER-microtubule bridge: Reticulon 17 links microtubules with ER network organisation in plants.The Plant journal : for cell and molecular biology · 2026Article
- Plant Golgi and GECCO: The Universality of ER-Golgi Interface.Sub-cellular biochemistry · 2026Review
- Inhibition of Sterol Biosynthesis Alters Tubulin Association with Detergent-Insoluble Membranes and Affects Microtubule Organization in Pollen Tubes ofPlants (Basel, Switzerland) · 2025Article
- Plant plastids: from evolutionary origins to functional specialization and organelle interactions.Journal of experimental botany · 2025Review
- The mutual influence of microtubules and the cortical ER on their coordinated organisation.Journal of microscopy · 2025Article
- Proximity driven plastid-nucleus relationships are facilitated by tandem plastid-ER dynamics.Journal of experimental botany · 2024Article
- Logistics of defense: The contribution of endomembranes to plant innate immunity.The Journal of cell biology · 2024Review
- Organelle Interactions in Plant Cells.Results and problems in cell differentiation · 2024Review
- Electron Microscopy Techniques for 3D Plant ER Imaging.Methods in molecular biology (Clifton, N.J.) · 2024Article
- The plasma membrane - cell wall nexus in plant cells: focus on the Hechtian structure.Cell surface (Amsterdam, Netherlands) · 2023Review
- Manipulation of the Cellular Membrane-Cytoskeleton Network for RNA Virus Replication and Movement in Plants.Viruses · 2023Review
- A glossary of plant cell structures: Current insights and future questions.The Plant cell · 2022Review
- The legacy of kinesins in the pollen tube 30 years later.Cytoskeleton (Hoboken, N.J.) · 2022Review
- A novel automated image analysis pipeline for quantifying morphological changes to the endoplasmic reticulum in cultured human cells.BMC bioinformatics · 2021Article
- Maintaining the structural and functional homeostasis of the plant endoplasmic reticulum.Developmental cell · 2021Review
- IntEResting structures: formation and applications of organized smooth endoplasmic reticulum in plant cells.Plant physiology · 2021Article
- Organelle extensions in plant cells.Plant physiology · 2021Article
- Armadillo-repeat kinesin1 interacts with Arabidopsis atlastin RHD3 to move ER with plus-end of microtubules.Nature communications · 2020Article
- The plant endoplasmic reticulum: an organized chaos of tubules and sheets with multiple functions.Journal of microscopy · 2020Review
- Spastin mutations impair coordination between lipid droplet dispersion and reticulum.PLoS genetics · 2020Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The endoplasmic reticulum (ER) is a network of tubules and sheet-like structures in eukaryotic cells. Some ER tubules dynamically change their morphology, and others form stable structures. In plants, it has been thought that the ER tubule extension is driven by the actin-myosin machinery. Here, we show that microtubules also contribute to the ER tubule extension with an almost 20-fold slower rate than the actin filament-based ER extension. Treatment with the actin-depolymerizing drug Latrunculin B made it possible to visualize the slow extension of the ER tubules in transgenic Arabidopsis (Arabidopsis thaliana) plants expressing ER-targeted green fluorescent protein. The ER tubules elongated along microtubules in both directions of microtubules, which have a distinct polarity. This feature is similar to the kinesin- or dynein-driven ER tubule extension in animal cells. In contrast to the animal case, ER tubules elongating with the growing microtubule ends were not observed in Arabidopsis. We also found the spots where microtubules are stably colocalized with the ER subdomains during long observations of 1,040 s, suggesting that cortical microtubules contribute to provide ER anchoring points. The anchoring points acted as the branching points of the ER tubules, resulting in the formation of multiway junctions. The density of the ER tubule junction positively correlated with the microtubule density in both elongating cells and mature cells of leaf epidermis, showing the requirement of microtubules for formation of the complex ER network. Taken together, our findings show that plants use microtubules for ER anchoring and ER tubule extension, which establish fine network structures of the ER within the cell.
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