Evidence map›Paper›PMID 25367792›Full record

ArticleJournal of Zhejiang University. Science. B2014

Development of 107 SSR markers from whole genome shotgun sequences of Chinese bayberry (Myrica rubra) and their application in seedling identification.

Hui-min Jia, Yu-tong Shen, Yun Jiao, Guo-yun Wang, Xiao Dong, Hui-juan Jia, Fang Du, Sen-miao Liang, Chao-chao Zhou, Wei-hua Mao and 1 more

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Article in Journal of Zhejiang University. Science. B, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

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6citing papers in PubMed
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3 · Its place in the literature

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6 citing papers in PubMed.

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5 · Who and what money

Authors and funding

11 authors.

Hui-min JiaDepartment of Horticulture, Zhejiang University, Hangzhou 310058, China; Fruit Research Institute, Yuyao 315400, China; Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; Yuyao Agricultural and Forestry Bureau, Yuyao 315400, China; Bio-Macromolecules Analysis Lab, Analysis Center of Agrobiology and Environmental Sciences, Zhejiang University, Hangzhou 310058, China.
Yu-tong Shen
Yun Jiao
Guo-yun Wang
Xiao Dong
Hui-juan Jia
Fang Du
Sen-miao Liang
Chao-chao Zhou
Wei-hua Mao
Zhong-shan Gao

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Chinese bayberry (Myrica rubra Sieb. et Zucc.) is one of the important subtropical fruit crops native to the South of China and Asian countries. In this study, 107 novel simple sequence repeat (SSR) molecular markers, a powerful tool for genetic diversity studies, cultivar identification, and linkage map construction, were developed and characterized from whole genome shotgun sequences. M13 tailing for forward primers was applied as a simple method in different situations. In total, 828 alleles across 45 accessions were detected, with an average of 8 alleles per locus. The number of effective alleles ranged from 1.22 to 10.41 with an average of 4.08. The polymorphic information content (PIC) varied from 0.13 to 0.89, with an average of 0.63. Moreover, these markers could also be amplified in their related species Myrica cerifera (syn. Morella cerifera) and Myrica adenophora. Seventy-eight SSR markers can be used to produce a genetic map of a cross between 'Biqi' and 'Dongkui'. A neighbor-joining (NJ) tree was constructed to assess the genetic relationships among accessions, and the elite accessions 'Y2010-70', 'Y2012-140', and 'Y2012-145', were characterized as potential new genotypes for cultivation.

Indexed as

Base SequenceChromosome MappingGenetic VariationGenome, PlantMicrosatellite RepeatsMolecular Sequence DataMyricaSeedlingsSequence Analysis, DNAChinese bayberryGenetic diversitySimple sequence repeat (SSR)

Identifiers

PMID25367792
PMCPMC4228512

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