Evidence map›Paper›PMID 25366827›Full record

ReviewPhotosynthesis research2015

Live-cell imaging of cyanobacteria.

Rayka Yokoo, Rachel D Hood, David F Savage

Abstract readReview
PubMed Publisher
In one paragraph

Review in Photosynthesis research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. pSHDY: A New Tool for Genetic Engineering of Cyanobacteria.Methods in molecular biology (Clifton, N.J.) · 2022
    Article
  8. Review
  9. Article
  10. Review
  11. Article
  12. Review
  13. Article
  14. Article
  15. Review
  16. The stringent response regulates adaptation to darkness in the cyanobacterium Synechococcus elongatus.Proceedings of the National Academy of Sciences of the United States of America · 2016
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Rayka YokooDepartment of Molecular & Cell Biology, University of California, Berkeley, Berkeley, CA, 94720, USA.
Rachel D HoodDepartment of Molecular & Cell Biology, University of California, Berkeley, Berkeley, CA, 94720, USA.
David F SavageDepartment of Molecular & Cell Biology, University of California, Berkeley, Berkeley, CA, 94720, USA. dsavage@berkeley.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cyanobacteria are a diverse bacterial phylum whose members possess a high degree of ultrastructural organization and unique gene regulatory mechanisms. Unraveling this complexity will require the use of live-cell fluorescence microscopy, but is impeded by the inherent fluorescent background associated with light-harvesting pigments and the need to feed photosynthetic cells light. Here, we outline a roadmap for overcoming these challenges. Specifically, we show that although basic cyanobacterial biology creates challenging experimental constraints, these restrictions can be mitigated by the careful choice of fluorophores and microscope instrumentation. Many of these choices are motivated by recent successful live-cell studies. We therefore also highlight how live-cell imaging has advanced our understanding of bacterial microcompartments, circadian rhythm, and the organization and segregation of the bacterial nucleoid.

Indexed as

Bacteriological TechniquesCircadian RhythmCyanobacteriaEquipment DesignFluorescent DyesImage Processing, Computer-AssistedMicroscopyMicroscopy, FluorescencePhotosynthesisPigments, BiologicalPolyploidyFluorescent DyesPigments, BiologicalCarboxysomeCircadian rhythmCyanobacteriaLive-cell microscopySynechococcus

Identifiers

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.