ArticleJournal of industrial microbiology & biotechnology2015
Overexpression of specific proton motive force-dependent transporters facilitate the export of surfactin in Bacillus subtilis.
Article in Journal of industrial microbiology & biotechnology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 2 of them syntheses that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed, 2 syntheses or guidelines pooled it, 75 citations in OpenAlex.
- Advancements in the Application of Ribosomally Synthesized and Post-Translationally Modified Peptides (RiPPs).Biomolecules · 2024Pooled it
- The Surfactin-Like Lipopeptides FromFrontiers in bioengineering and biotechnology · 2021Pooled it
- Secondary Metabolite Biosynthesis and Regulatory Role of Sucrose inMicroorganisms · 2026Review
- Metabolic engineering of Bacillus subtilis for high-yield surfactin production.Biotechnology for biofuels and bioproducts · 2026Article
- The TerC family metal chaperone MeeY enables surfactin export inJournal of bacteriology · 2025Article
- Development of a microbial dewaxing agent using three spore forming bacteria.Bioresources and bioprocessing · 2024Article
- An Integrated Pipeline and Overexpression of a Novel Efflux Transporter, YoeA, Significantly Increases Plipastatin Production inFoods (Basel, Switzerland) · 2024Article
- Strategies for improving fengycin production: a review.Microbial cell factories · 2024Review
- Improving Surfactin Production inMicroorganisms · 2024Article
- Modular metabolic engineering of Bacillus amyloliquefaciens for high-level production of green biosurfactant iturin A.Applied microbiology and biotechnology · 2024Article
- Research advances in the identification of regulatory mechanisms of surfactin production by Bacillus: a review.Microbial cell factories · 2024Review
- Antimicrobial peptides from Bacillus spp. and strategies to enhance their yield.Applied microbiology and biotechnology · 2023Review
- A review on surfactin: molecular regulation of biosynthesis.Archives of microbiology · 2023Review
- Chemical structure, properties and potential applications of surfactin, as well as advanced strategies for improving its microbial production.AIMS microbiology · 2023Review
- LC-MS and Transcriptome Analysis of Lipopeptide Biosynthesis byMolecules (Basel, Switzerland) · 2022Article
- Biological Control ofMicroorganisms · 2022Review
- Enhanced production of iturin A by strengthening fatty acid synthesis modules inFrontiers in bioengineering and biotechnology · 2022Article
- Bacillus sp.: A Remarkable Source of Bioactive Lipopeptides.Advances in biochemical engineering/biotechnology · 2022Article
- Micelle morphology observation method of lipopeptide by negative-staining-based transmission electron microscopy.Biotechnology notes (Amsterdam, Netherlands) · 2022Article
- Microbial production of small peptide: pathway engineering and synthetic biology.Microbial biotechnology · 2021Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A novel surfactin producer, Bacillus subtilis THY-7, was isolated from soil. Using liposomes and transmembrane transport inhibitors, the surfactin efflux in THY-7 was determined to be mainly dependent on proton motive force (PMF), not ATP hydrolysis. YcxA, KrsE and YerP, three putative lipopeptide transporters with PMF as energy source, were then highlighted in this work. A mutant YcxA named as YcxAmt, with 2 transmembrane helices deletion due to a code-shift mutation of the encoding gene, was identified in THY-7. This truncated YcxAmt was confirmed unable to transfer surfactin; on the contrary, overexpression of the natural full-lengthYcxA enhanced the secretion of surfactin by 89 %. KrsE, a putative kurstakin transporter, was found also capable of transporting surfactin. Overexpression of KrsE increased the production of surfactin by 52 %. In the culture of YerP-overexpressing strain at 24 h, surfactin titer reached 1.58 g L(-1), which was 145 % higher than that of the control. This indicated that YerP acted as the major surfactin exporter in B. subtilis THY-7.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.