ArticleMicrobial cell factories2014
Metabolomic basis of laboratory evolution of butanol tolerance in photosynthetic Synechocystis sp. PCC 6803.
Article in Microbial cell factories, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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21 citing papers in PubMed, 62 citations in OpenAlex.
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- Understanding and engineering alcohol-tolerant bacteria using OMICS technology.World journal of microbiology & biotechnology · 2018Review
- Microalgae for the production of lipid and carotenoids: a review with focus on stress regulation and adaptation.Biotechnology for biofuels · 2018Review
- Growth and lipid accumulation by different nutrients in the microalgaBiotechnology for biofuels · 2018Article
- Adaptive laboratory evolution of cadmium tolerance inBiotechnology for biofuels · 2018Article
- Mechanism and enhancement of lipid accumulation in filamentous oleaginous microalgaeBiotechnology for biofuels · 2018Article
- Progress and perspectives on improving butanol tolerance.World journal of microbiology & biotechnology · 2017Review
- Functional Diversity of Transcriptional Regulators in the CyanobacteriumFrontiers in microbiology · 2017Article
- A novel small RNA CoaR regulates coenzyme A biosynthesis and tolerance ofBiotechnology for biofuels · 2017Article
- Algal Cell Factories: Approaches, Applications, and Potentials.Marine drugs · 2016Review
- Fatty acid and metabolomic profiling approaches differentiate heterotrophic and mixotrophic culture conditions in a microalgal food supplement 'Euglena'.BMC biotechnology · 2016Article
- Proteomic and metabolomic analyses reveal metabolic responses to 3-hydroxypropionic acid synthesized internally in cyanobacteriumBiotechnology for biofuels · 2016Article
- Cyanobacteria: Photoautotrophic Microbial Factories for the Sustainable Synthesis of Industrial Products.BioMed research international · 2015Review
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRecent efforts demonstrated the potential application of cyanobacteria as a "microbial cell factory" to produce butanol directly from CO2. However, cyanobacteria have very low tolerance to the toxic butanol, which limits the economic viability of this renewable system.
resultsThrough a long-term experimental evolution process, we achieved a 150% increase of the butanol tolerance in a model cyanobacterium Synechocystis sp. PCC 6803 after a continuous 94 passages for 395 days in BG11 media amended with gradually increased butanol concentration from 0.2% to 0.5% (v/v). To decipher the molecular mechanism responsible for the tolerance increase, we employed an integrated GC-MS and LC-MS approach to determine metabolomic profiles of the butanol-tolerant Synechocystis strains isolated from several stages of the evolution, and then applied PCA and WGCNA network analyses to identify the key metabolites and metabolic modules related to the increased tolerance. The results showed that unstable metabolites of 3-phosphoglyceric acid (3PG), D-fructose 6-phosphate (F6P), D-glucose 6-phosphate (G6P), NADPH, phosphoenolpyruvic acid (PEP), D-ribose 5-phosphate (R5P), and stable metabolites of glycerol, L-serine and stearic acid were differentially regulated during the evolution process, which could be related to tolerance increase to butanol in Synechocystis.
conclusionsThe study provided the first time-series description of the metabolomic changes related to the gradual increase of butanol tolerance, and revealed a metabolomic basis important for rational tolerance engineering in Synechocystis.
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