ArticleThe Journal of cell biology2014
A fluorescent tagging approach in Drosophila reveals late endosomal trafficking of Notch and Sanpodo.
Article in The Journal of cell biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 54 citations in OpenAlex.
- Jagged-mediated lateral induction patterns Notch3 signaling within adult neural stem cell populations.Nature communications · 2026Article
- Novel determinants of NOTCH1 trafficking and signaling in breast epithelial cells.Life science alliance · 2025Article
- Spatio-Temporal Regulation of Notch Activation in Asymmetrically Dividing Sensory Organ Precursor Cells inCells · 2024Review
- Enhanced Photodynamic Therapy Synergizing with Inhibition of Tumor Neutrophil Ferroptosis Boosts Anti-PD-1 Therapy of Gastric Cancer.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- The meaning of ubiquitylation of the DSL ligand Delta for the development of Drosophila.BMC biology · 2023Article
- Review
- Parkinson disease-associated Leucine-rich repeat kinase regulates UNC-104-dependent axonal transport of Arl8-positive vesicles iniScience · 2022Article
- A combination of Notch signaling, preferential adhesion and endocytosis induces a slow mode of cell intercalation in the Drosophila retina.Development (Cambridge, England) · 2021Article
- Intracellular trafficking of Notch orchestrates temporal dynamics of Notch activity in the fly brain.Nature communications · 2021Article
- The Clathrin adaptor AP-1 and Stratum act in parallel pathways to control Notch activation inDevelopment (Cambridge, England) · 2021Article
- Lethal (2) giant discs (Lgd)/CC2D1 is required for the full activity of the ESCRT machinery.BMC biology · 2020Article
- Phosphatidic acid increases Notch signalling by affecting Sanpodo trafficking during Drosophila sensory organ development.Scientific reports · 2020Article
- Optogenetic inhibition of Delta reveals digital Notch signalling output during tissue differentiation.EMBO reports · 2019Article
- The Late Endosomal Pathway Regulates the Ciliary Targeting of Tetraspanin Protein Peripherin 2.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
- Article
- AKAP200 promotes Notch stability by protecting it from Cbl/lysosome-mediated degradation in Drosophila melanogaster.PLoS genetics · 2018Article
- Adaptor proteins NUMB and NUMBL promote cell cycle withdrawal by targeting ERBB2 for degradation.The Journal of clinical investigation · 2017Article
- Boudin trafficking reveals the dynamic internalisation of specific septate junction components in Drosophila.PloS one · 2017Article
- Variant Exported Blood-Stage Proteins Encoded by Plasmodium Multigene Families Are Expressed in Liver Stages Where They Are Exported into the Parasitophorous Vacuole.PLoS pathogens · 2016Article
- Regulation of Dense-Core Granule Replenishment by Autocrine BMP Signalling in Drosophila Secondary Cells.PLoS genetics · 2016Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Signaling and endocytosis are highly integrated processes that regulate cell fate. In the Drosophila melanogaster sensory bristle lineages, Numb inhibits the recycling of Notch and its trafficking partner Sanpodo (Spdo) to regulate cell fate after asymmetric cell division. In this paper, we have used a dual GFP/Cherry tagging approach to study the distribution and endosomal sorting of Notch and Spdo in living pupae. The specific properties of GFP, i.e., quenching at low pH, and Cherry, i.e., slow maturation time, revealed distinct pools of Notch and Spdo: cargoes exhibiting high GFP/low Cherry fluorescence intensities localized mostly at the plasma membrane and early/sorting endosomes, whereas low GFP/high Cherry cargoes accumulated in late acidic endosomes. These properties were used to show that Spdo is sorted toward late endosomes in a Numb-dependent manner. This dual-tagging approach should be generally applicable to study the trafficking dynamics of membrane proteins in living cells and tissues.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.