ArticleJournal of dental research2015
Iron binding modulates candidacidal properties of salivary histatin 5.
Article in Journal of dental research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
22 citing papers in PubMed, 41 citations in OpenAlex.
- Review
- Saliva Protein Genes in Humans were Shaped During Primate Evolution.Genome biology and evolution · 2025Article
- Emergence of saliva protein genes in the secretory calcium-binding phosphoprotein (SCPP) locus and accelerated evolution in primates.bioRxiv : the preprint server for biology · 2024Article
- Article
- Anti-biofilm effect of salivary histatin 5 on Porphyromonas gingivalis.Applied microbiology and biotechnology · 2023Article
- Salivary Antimicrobial Peptide Histatin-5 Does Not Display Zn(II)-Dependent or -Independent Activity against Streptococci.ACS infectious diseases · 2023Article
- Zinc Binding Inhibits Cellular Uptake and Antifungal Activity of Histatin-5 inACS infectious diseases · 2022Article
- Antimicrobial Peptides with Anti-International journal of molecular sciences · 2022Review
- A Novel Role for Histatin 5 in Combination with Zinc to Promote Commensalism inPathogens (Basel, Switzerland) · 2021Article
- Influence of iron binding in the structural stability and cellular internalization of bovine lactoferrin.Heliyon · 2021Article
- Histatin 5 Metallopeptides and Their Potential againstBiomolecules · 2021Review
- Zinc Binding by Histatin 5 Promotes Fungicidal Membrane Disruption inJournal of fungi (Basel, Switzerland) · 2020Article
- Bio- and Nanotechnology as the Key for Clinical Application of Salivary Peptide Histatin: A Necessary Advance.Microorganisms · 2020Review
- Metal Binding Antimicrobial Peptides in Nanoparticle Bio-functionalization: New Heights in Drug Delivery and Therapy.Probiotics and antimicrobial proteins · 2020Review
- Antimicrobial Peptides: Classification, Design, Application and Research Progress in Multiple Fields.Frontiers in microbiology · 2020Review
- Dynamical Oligomerisation of Histidine Rich Intrinsically Disordered ProteinS Is Regulated through Zinc-Histidine Interactions.Biomolecules · 2019Article
- Iron Chelator Deferasirox ReducesAntimicrobial agents and chemotherapy · 2019Article
- Specific Histidine Residues Confer Histatin Peptides with Copper-Dependent Activity against Candida albicans.Biochemistry · 2017Article
- Structural Characterization of Histatin 5-Spermidine Conjugates: A Combined Experimental and Theoretical Study.Journal of chemical information and modeling · 2017Article
- Human Salivary Protein Histatin 5 Has Potent Bactericidal Activity against ESKAPE Pathogens.Frontiers in cellular and infection microbiology · 2017Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Salivary protein histatin 5 (Hst 5) is fungicidal toward Candida albicans, the causative agent of oropharyngeal candidiasis. However, its activity in saliva is compromised by salivary protease-mediated degradation and interaction with salivary salts. Hst 5 has also been shown to bind various metals in saliva-namely, Zn, Cu, and Ni. Surprisingly, interactions of Hst 5 with Fe have not been studied, although iron is one of the most abundant metals present in saliva. Using circular dichroism, we show that Hst 5 can bind up to 10 equivalents of iron as measured by loss of its alpha-helical secondary structure that is normally observed for it in trifluoroethylene. A significant decrease in the candidacidal ability of Hst 5 was observed upon iron binding, with increasing iron concentrations being inversely proportional to Hst 5 killing activity. Binding assays showed that the decrease in killing was likely a result of reduced binding (10-fold reduction) of Fe-Hst 5 to C. albicans cells. Protease stability analysis showed that Fe-Hst 5 was completely resistant to trypsin digestion. In contrast, zinc binding had limited effects on Hst 5 fungicidal activity or protease susceptibility. RNA sequencing results identified changes in iron uptake genes in Hst 5-treated C. albicans cells. Our findings thus suggest that consequences of Hst 5 binding iron not only affect candidacidal ability and proteolyic stability of Hst 5, but may also contribute to a novel killing mechanism involving interference with cellular iron metabolism.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.