ArticlePloS one2014
Quantitative spatial and temporal analysis of fluorescein angiography dynamics in the eye.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 20 citations in OpenAlex.
- Automated Assessment of Retinal Vascular Integrity Across Species.Investigative ophthalmology & visual science · 2025Article
- Synchronized strobed phase contrast and fluorescence microscopy: the interlaced standard reimagined.Optics express · 2023Article
- Fluorescence Angiography with Dual Fluorescence for the Early Detection and Longitudinal Quantitation of Vascular Leakage in Retinopathy.Biomedicines · 2023Article
- Microglia: The breakthrough to treat neovascularization and repair blood-retinal barrier in retinopathy.Frontiers in molecular neuroscience · 2023Review
- In vivo Vascular Injury Readouts in Mouse Retina to Promote Reproducibility.Journal of visualized experiments : JoVE · 2022Article
- Fractalkine-induced microglial vasoregulation occurs within the retina and is altered early in diabetic retinopathy.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Quantification of retinal blood leakage in fundus fluorescein angiography in a retinal angiogenesis model.Scientific reports · 2021Article
- En-Face Optical Coherence Tomography Angiography for Longitudinal Monitoring of Retinal Injury.Applied sciences (Basel, Switzerland) · 2019Article
- A Naturally Fluorescent Mgp Transgenic Mouse for Angiogenesis and Glaucoma Longitudinal Studies.Investigative ophthalmology & visual science · 2018Article
- Small GTPase ARF6 controls VEGFR2 trafficking and signaling in diabetic retinopathy.The Journal of clinical investigation · 2017Article
- In Vivo Imaging of Retinal Hypoxia Using HYPOX-4-Dependent Fluorescence in a Mouse Model of Laser-Induced Retinal Vein Occlusion (RVO).Investigative ophthalmology & visual science · 2017Article
- Retinal and Cortical Blood Flow Dynamics Following Systemic Blood-Neural Barrier Disruption.Frontiers in neuroscience · 2017Article
- In Vivo Angiography Quantifies Oxygen-Induced Retinopathy Vascular Recovery.Optometry and vision science : official publication of the American Academy of Optometry · 2016Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeWe describe a novel approach to analyze fluorescein angiography to investigate fluorescein flow dynamics in the rat posterior retina as well as identify abnormal areas following laser photocoagulation.
methodsExperiments were undertaken in adult Long Evans rats. Using a rodent retinal camera, videos were acquired at 30 frames per second for 30 seconds following intravenous introduction of sodium fluorescein in a group of control animals (n = 14). Videos were image registered and analyzed using principle components analysis across all pixels in the field. This returns fluorescence intensity profiles from which, the half-rise (time to 50% brightness), half-fall (time for 50% decay) back to an offset (plateau level of fluorescence). We applied this analysis to video fluorescein angiography data collected 30 minutes following laser photocoagulation in a separate group of rats (n = 7).
resultsPixel-by-pixel analysis of video angiography clearly delineates differences in the temporal profiles of arteries, veins and capillaries in the posterior retina. We find no difference in half-rise, half-fall or offset amongst the four quadrants (inferior, nasal, superior, temporal). We also found little difference with eccentricity. By expressing the parameters at each pixel as a function of the number of standard deviation from the average of the entire field, we could clearly identify the spatial extent of the laser injury.
conclusionsThis simple registration and analysis provides a way to monitor the size of vascular injury, to highlight areas of subtle vascular leakage and to quantify vascular dynamics not possible using current fluorescein angiography approaches. This can be applied in both laboratory and clinical settings for in vivo dynamic fluorescent imaging of vasculature.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.