ArticlePloS one2014
Immunization with Hypoallergens of shrimp allergen tropomyosin inhibits shrimp tropomyosin specific IgE reactivity.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 72 citations in OpenAlex.
- Recent insights for fish allergens: novel processing techniques and detection technology.Journal of the science of food and agriculture · 2026Review
- Next-Generation Allergen-Specific Immunotherapy for Food Allergy.Clinical reviews in allergy & immunology · 2025Review
- Article
- Advances in Shellfish Allergy Therapy: From Current Approaches to Future Strategies.Clinical reviews in allergy & immunology · 2025Review
- Molecular Allergology: Epitope Discovery and Its Application for Allergen-Specific Immunotherapy of Food Allergy.Clinical reviews in allergy & immunology · 2025Review
- Recent Advances of Processing and Detection Techniques on Crustacean Allergens: A Review.Foods (Basel, Switzerland) · 2025Review
- Shrimp Extract Exacerbates Allergic Immune Responses in Mice: Implications on Clinical Diagnosis of Shellfish Allergy.Clinical reviews in allergy & immunology · 2024Article
- Diagnosis and management of shrimp allergy.Frontiers in allergy · 2024Review
- IgE Mediated Shellfish Allergy in Children-A Review.Nutrients · 2023Review
- IgE-Mediated Shellfish Allergy in Children.Nutrients · 2023Review
- Comprehending the allergen repertoire of shrimp for precision molecular diagnosis of shrimp allergy.Allergy · 2022Article
- Reduction in Allergenicity and Induction of Oral Tolerance of Glycated Tropomyosin from Crab.Molecules (Basel, Switzerland) · 2022Article
- Pentraxin 3 Facilitates Shrimp-Allergic Responses in IgE-Activated Mast Cells.Journal of immunology research · 2022Article
- IgE and IgG4 responses to shrimp allergen tropomyosin and its epitopes in patients from coastal areas of northern China.Molecular medicine reports · 2020Article
- Overcoming Shellfish Allergy: How Far Have We Come?International journal of molecular sciences · 2020Review
- Modulating Shrimp Tropomyosin-Mediated Allergy: Hypoallergen DNA Vaccines Induce Regulatory T Cells to Reduce Hypersensitivity in Mouse Model.International journal of molecular sciences · 2019Article
- Review
- Immunotherapy of Food Allergy: a Comprehensive Review.Clinical reviews in allergy & immunology · 2019Review
- Conservation Analysis of B-Cell Allergen Epitopes to Predict Clinical Cross-Reactivity Between Shellfish and Inhalant Invertebrate Allergens.Frontiers in immunology · 2019Article
- Screening and identification of mimotopes of the major shrimp allergen tropomyosin using one-bead-one-compound peptide libraries.Cellular & molecular immunology · 2017Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Designer proteins deprived of its IgE-binding reactivity are being sought as a regimen for allergen-specific immunotherapy. Although shrimp tropomyosin (Met e 1) has long been identified as the major shellfish allergen, no immunotherapy is currently available. In this study, we aim at identifying the Met e 1 IgE epitopes for construction of hypoallergens and to determine the IgE inhibitory capacity of the hypoallergens. IgE-binding epitopes were defined by three online computational models, ELISA and dot-blot using sera from shrimp allergy patients. Based on the epitope data, two hypoallergenic derivatives were constructed by site-directed mutagenesis (MEM49) and epitope deletion (MED171). Nine regions on Met e 1 were defined as the major IgE-binding epitopes. Both hypoallergens MEM49 and MED171 showed marked reduction in their in vitro reactivity towards IgE from shrimp allergy patients and Met e 1-sensitized mice, as well as considerable decrease in induction of mast cell degranulation as demonstrated in passive cutaneous anaphylaxis assay. Both hypoallergens were able to induce Met e 1-recognizing IgG antibodies in mice, specifically IgG2a antibodies, that strongly inhibited IgE from shrimp allergy subjects and Met e 1-sensitized mice from binding to Met e 1. These results indicate that the two designer hypoallergenic molecules MEM49 and MED171 exhibit desirable preclinical characteristics, including marked reduction in IgE reactivity and allergenicity, as well as ability to induce blocking IgG antibodies. This approach therefore offers promises for development of immunotherapeutic regimen for shrimp tropomyosin allergy.
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