ArticlePloS one2014
Factors influencing the measurement of plasma/serum surfactant protein D levels by ELISA.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Study on the Impact of Cross-Reactive Antibodies Induced by SARS-COV-2 Infection on the False-Positive Rate of HIV Serological Testing.Indian journal of hematology & blood transfusion : an official journal of Indian Society of Hematology and Blood Transfusion · 2026Article
- Surfactant protein-D is an independent predictor of all-cause mortality in men with peripheral artery disease diagnosed by population-based screening.Frontiers in cardiovascular medicine · 2025Article
- Pulmonary surfactants and the respiratory-renal connection in steroid-sensitive nephrotic syndrome of childhood.iScience · 2022Article
- Microproteomics with microfluidic-based cell sorting: Application to 1000 and 100 immune cells.Proteomics · 2017Article
- Evaluation and Validation of the Detection of soluble Triggering Receptor Expressed on Myeloid Cells 1 by Enzyme-linked immunosorbent Assay.Scientific reports · 2015Article
- Synthesis of Phospholipid-Protein Conjugates as New Antigens for Autoimmune Antibodies.Molecules (Basel, Switzerland) · 2015Article
- Biomarkers of lung injury in cardiothoracic surgery.Disease markers · 2015Review
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Authors and funding
2 authors.
Funding
Abstract
backgroundExtensive variations in human surfactant protein D (SP-D) levels in circulation as measured by ELISA exist in the published literature. In order to determine the source of these variations, factors influencing the measurement by ELISA were explored. MATERIALS AND
methodsPeripheral blood from healthy individuals was collected into various vacutainers during the same blood draw. Recombinant SP-D was diluted into different matrices and used for a standard curve. Samples were analyzed by capture ELISA using one of two distinct detection antibodies.
resultsThe type of matrix had some effects on detection of recombinant SP-D. The type of anticoagulant used and dilution factor had very little effect, except for in plasma collected in EDTA vacutainers. The extent of variation in published values seemed to be due to the ELISA configuration employed, and, in agreement with this, we found that by switching the detection antibody, there was a 50% decrease in the extrapolated SP-D value of serum and plasma samples. Storage of samples resulted in slight changes in measured SP-D levels.
conclusionsThe ELISA configuration employed to measure circulating levels of SP-D has a significant effect on the extrapolated values. In both configurations tested, the use of EDTA as a coagulant resulted in inconsistent values, and we, therefore, suggest the avoidance of this anticoagulant when assaying for SP-D by ELISA. While the demonstrated effects of several factors on measurement of SP-D may not account for all the disparities amongst the previous studies, they stress that variations in methodologies for measuring the same protein can result in very inconsistent results.
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