Evidence map›Paper›PMID 25365206›Full record

ArticleLaboratory investigation; a journal of technical methods and pathology2014

Nestin depletion induces melanoma matrix metalloproteinases and invasion.

Chung-Wei Lee, Qian Zhan, Cecilia Lezcano, Markus H Frank, John Huang, Allison R Larson, Jennifer Y Lin, Marilyn T Wan, Ping-I Lin, Jie Ma and 4 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Laboratory investigation; a journal of technical methods and pathology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
0.4field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 14 citations in OpenAlex.

  1. PRAME Expression in Melanoma is Negatively Regulated by TET2-Mediated DNA Hydroxymethylation.Laboratory investigation; a journal of technical methods and pathology · 2025
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  5. ATF-3 expression inhibits melanoma growth by downregulating ERK and AKT pathways.Laboratory investigation; a journal of technical methods and pathology · 2021
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 3 institutions in 1 country.

Chung-Wei LeeDepartment of Pathology, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA.
Qian ZhanDepartment of Pathology, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA.
Cecilia LezcanoDepartment of Pathology, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA.
Markus H FrankTransplantation Research Center, Children's Hospital and Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
John Huang1] Department of Pathology, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA [2] Department of Surgery, National Taiwan University Hospital, Taipei, Taiwan.
Allison R LarsonDepartment of Pathology, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA.
Jennifer Y LinDepartment of Dermatology, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA.
Marilyn T Wan1] Department of Pathology, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA [2] Department of Dermatology, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA.
Ping-I LinDivision of Biostatistics and Epidemiology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA.
Jie MaTransplantation Research Center, Children's Hospital and Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
Sonja KleffelDepartment of Dermatology, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA.
Tobias SchattonDepartment of Dermatology, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA.
Christine G LianDepartment of Pathology, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA.
George F MurphyDepartment of Pathology, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA.
Brigham and Women's Hospital · USHarvard University · USCincinnati Children's Hospital Medical Center · US

Funding

Harvard Clinical and Translational Science Center (UL1)UL1TR000170 · NCATS · HARVARD MEDICAL SCHOOL · PI NADLER, LEE MARSHALL · 2012 to 2013
$31.4M
Targeting Raf in Melanoma: Mechanisms for Effect & Resistance & Opportunities forP50CA093683 · NCI · BRIGHAM AND WOMEN'S HOSPITAL · PI MARKUS, FRANK · 2001 to 2012
$25.8M
VASCULAR, PULMONARY AND RENAL INJURYT32HL007627 · NHLBI · BRIGHAM AND WOMEN'S HOSPITAL · PI GIMBRONE, MICHAEL A, MITCHELL, RICHARD N · 1985 to 2019
$12.7M
DERMATOLOGY TRAINING GRANTT32AR007098 · NIAMS · HARVARD UNIVERSITY (MEDICAL SCHOOL) · PI THOMAS S. KUPPER · 1986 to 2026
$8.1M
Immunology of human malignant melanoma initiating cellsR01CA158467 · NCI · BOSTON CHILDREN'S HOSPITAL · PI FRANK, MARKUS H., HODI, FRANK S · 2012 to 2016
$1.9M
Melanoma Stem Cells, Vasculogenesis and Neoplastic ProgressionR01CA138231 · NCI · BRIGHAM AND WOMEN'S HOSPITAL · PI FRANK, MARKUS H. · 2008 to 2012
$1.7M
ABCB5 P-Glycoprotein in Cancer Multidrug ResistanceR01CA113796 · NCI · BOSTON CHILDREN'S HOSPITAL · PI FRANK, MARKUS H. · 2006 to 2010
$1.5M
ABCB5+ Dermal Stem Cells In Regenerative Wound HealingI01RX000989 · VA · VA BOSTON HEALTH CARE SYSTEM · PI FRANK, NATASHA Y · 2015 to 2017
–
NCATS NIH HHS UL1 TR000170NCI NIH HHS 5P50CA093683-09NCI NIH HHS P50 CA093683NCI NIH HHS R01 CA113796NCI NIH HHS R01 CA138231NCI NIH HHS R01 CA158467NHLBI NIH HHS 5T32HL7627-29NHLBI NIH HHS T32 HL007627NIAMS NIH HHS 2T32AR7098-37NIAMS NIH HHS T32 AR007098NIAMS NIH HHS T32AR007098-38RRD VA I01 RX000989
6 · The paper itself

Abstract

Matrix metalloproteinases (MMPs) are key biological mediators of processes as diverse as wound healing, embryogenesis, and cancer progression. Although MMPs may be induced through multiple signaling pathways, the precise mechanisms for their regulation in cancer are incompletely understood. Because cytoskeletal changes are known to accompany MMP expression, we sought to examine the potential role of the poorly understood cytoskeletal protein, nestin, in modulating melanoma MMPs. Nestin knockdown (KD) upregulated the expression of specific MMPs and MMP-dependent invasion both through extracellular matrix barriers in vitro and in peritumoral connective tissue of xenografts in vivo. The development of three-dimensional melanospheres that in vitro partially recapitulate noninvasive tumorigenic melanoma growth was inhibited by nestin KD, although ECM invasion by aberrant melanospheres that did form was enhanced. Mechanistically, nestin KD-dependent melanoma invasion was associated with intracellular redistribution of phosphorylated focal adhesion kinase and increased melanoma cell responsiveness to transforming growth factor-beta, both implicated in pathways of melanoma invasion. The results suggest that the heretofore poorly understood intermediate filament, nestin, may serve as a novel mediator of MMPs critical to melanoma virulence.

Indexed as

AnimalsCell Line, TumorFemaleFocal Adhesion Protein-Tyrosine KinasesHumansMatrix MetalloproteinasesMelanomaMiceNeoplasm InvasivenessNestinTransforming Growth Factor betaFocal Adhesion Protein-Tyrosine KinasesMatrix MetalloproteinasesNES protein, humanNestinTransforming Growth Factor beta

Identifiers

PMID25365206
PMCPMC4419570
OpenAlexW2145363743

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.