Evidence map›Paper›PMID 25365202›Full record

ReviewLaboratory investigation; a journal of technical methods and pathology2015

Generation of pluripotent stem cells without the use of genetic material.

Akon Higuchi, Qing-Dong Ling, S Suresh Kumar, Murugan A Munusamy, Abdullah A Alarfaj, Yung Chang, Shih-Hsuan Kao, Ke-Chen Lin, Han-Chow Wang, Akihiro Umezawa

Open access · bronzeAbstract readReview
PubMed Publisher
In one paragraph

Review in Laboratory investigation; a journal of technical methods and pathology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.

0numbers the graph read from it
0cells of the map it votes in
32citing papers in PubMed
4.4field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

32 citing papers in PubMed, 73 citations in OpenAlex.

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  15. Prevention of tumor risk associated with the reprogramming of human pluripotent stem cells.Journal of experimental & clinical cancer research : CR · 2020
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  19. Histone Deacetylase Inhibitors as Anticancer Drugs.International journal of molecular sciences · 2017
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 6 institutions in 4 countries.

Akon Higuchi1] Department of Chemical and Materials Engineering, National Central University, Jhongli, Taiwan [2] Department of Botany and Microbiology, King Saud University, Riyadh, Saudi Arabia.
Qing-Dong Ling1] Institute of Systems Biology and Bioinformatics, National Central University, Jhongli, Taiwan [2] Cathay Medical Research Institute, Cathay General Hospital, Taipei, Taiwan.
S Suresh KumarDepartment of Medical Microbiology and Parasitology, Universities Putra Malaysia, Slangor, Malaysia.
Murugan A MunusamyDepartment of Botany and Microbiology, King Saud University, Riyadh, Saudi Arabia.
Abdullah A AlarfajDepartment of Botany and Microbiology, King Saud University, Riyadh, Saudi Arabia.
Yung ChangDepartment of Chemical Engineering, R&D Center for Membrane Technology, Chung Yuan Christian University, Jhongli, Taiwan.
Shih-Hsuan KaoDepartment of Chemical and Materials Engineering, National Central University, Jhongli, Taiwan.
Ke-Chen LinDepartment of Chemical and Materials Engineering, National Central University, Jhongli, Taiwan.
Han-Chow WangDepartment of Obstetrics and Gynecology, Hungchi Women and Children's Hospital, Jhongli, Taiwan.
Akihiro UmezawaDepartment of Reproduction, National Research Institute for Child Health and Development, Tokyo, Japan.
National Central University · TWKing Saud University · SAChung Yuan Christian University · TWNational Center For Child Health and Development · JPTaipei Municipal Women and Children's Hospital · TWUniversiti Putra Malaysia · MY

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Induced pluripotent stem cells (iPSCs) provide a platform to obtain patient-specific cells for use as a cell source in regenerative medicine. Although iPSCs do not have the ethical concerns of embryonic stem cells, iPSCs have not been widely used in clinical applications, as they are generated by gene transduction. Recently, iPSCs have been generated without the use of genetic material. For example, protein-induced PSCs and chemically induced PSCs have been generated by the use of small and large (protein) molecules. Several epigenetic characteristics are important for cell differentiation; therefore, several small-molecule inhibitors of epigenetic-modifying enzymes, such as DNA methyltransferases, histone deacetylases, histone methyltransferases, and histone demethylases, are potential candidates for the reprogramming of somatic cells into iPSCs. In this review, we discuss what types of small chemical or large (protein) molecules could be used to replace the viral transduction of genes and/or genetic reprogramming to obtain human iPSCs.

Indexed as

AnimalsGenetic TechniquesHumansMicePluripotent Stem Cells

Identifiers

PMID25365202
OpenAlexW2043039313

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.