ArticleNature materials2015
Designer amphiphilic proteins as building blocks for the intracellular formation of organelle-like compartments.
Article in Nature materials, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 95 citations in OpenAlex.
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- SNARE-Mediated Membrane Fusion Probed Using a Synthetic Organelle in the Living Bacterium.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Enzymatic Reaction Network-Driven Polymerization-Induced Transient Coacervation.Angewandte Chemie (International ed. in English) · 2024Article
- Hydrophobic mismatch drives self-organization of designer proteins into synthetic membranes.Nature communications · 2024Article
- DNA-guided self-assembly in living cells.iScience · 2023Review
- Strategies for Conditional Regulation of Proteins.JACS Au · 2023Review
- Impact of aromatic residues on the intrinsic disorder and transitional behaviour of model IDPs.Materials today. Bio · 2022Article
- Breaking the nanoparticle's dispersible limit via rotatable surface ligands.Nature communications · 2022Article
- Dynamic Structural Changes and Thermodynamics in Phase Separation Processes of an Intrinsically Disordered-Ordered Protein Model.Angewandte Chemie (International ed. in English) · 2022Article
- Purification of a Hydrophobic Elastin-Like Protein Toward Scale-Suitable Production of Biomaterials.Frontiers in bioengineering and biotechnology · 2022Article
- Spatiotemporal Dynamic Assembly/Disassembly of Organelle-Mimics Based on Intrinsically Disordered Protein-Polymer Conjugates.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
- Self-assembly of protein superstructures by physical interactions under cytoplasm-like conditions.Biophysical journal · 2021Article
- Artificial Organelles: Towards Adding or Restoring Intracellular Activity.Chembiochem : a European journal of chemical biology · 2021Review
- Synthetic biology as driver for the biologization of materials sciences.Materials today. Bio · 2021Review
- Formation of Biomolecular Condensates in Bacteria by Tuning Protein Electrostatics.ACS central science · 2020Article
- Formation and functionalization of membraneless compartments in Escherichia coli.Nature chemical biology · 2020Article
- De novo engineering of intracellular condensates using artificial disordered proteins.Nature chemistry · 2020Article
- Article
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nanoscale biological materials formed by the assembly of defined block-domain proteins control the formation of cellular compartments such as organelles. Here, we introduce an approach to intentionally 'program' the de novo synthesis and self-assembly of genetically encoded amphiphilic proteins to form cellular compartments, or organelles, in Escherichia coli. These proteins serve as building blocks for the formation of artificial compartments in vivo in a similar way to lipid-based organelles. We investigated the formation of these organelles using epifluorescence microscopy, total internal reflection fluorescence microscopy and transmission electron microscopy. The in vivo modification of these protein-based de novo organelles, by means of site-specific incorporation of unnatural amino acids, allows the introduction of artificial chemical functionalities. Co-localization of membrane proteins results in the formation of functionalized artificial organelles combining artificial and natural cellular function. Adding these protein structures to the cellular machinery may have consequences in nanobiotechnology, synthetic biology and materials science, including the constitution of artificial cells and bio-based metamaterials.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.