ArticleThe Journal of biological chemistry2014
Cellulose surface degradation by a lytic polysaccharide monooxygenase and its effect on cellulase hydrolytic efficiency.
Article in The Journal of biological chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 105 papers.
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Who cites it
105 citing papers in PubMed, 284 citations in OpenAlex.
- Reduced Processivity in a Chitobiohydrolase Enhances LPMO-Assisted Chitin Depolymerization.Biochemistry · 2026Article
- The Functional Characterization of an AA10 Lytic Polysaccharide Monooxygenase fromFoods (Basel, Switzerland) · 2025Article
- Influence of Expansion Pretreatment on the Structure Evolution and Reaction Chemistry during Oxidative Pyrolysis of Tobacco Biomass.ACS omega · 2025Article
- Functional characterization of two AA10 lytic polysaccharide monooxygenases from Cellulomonas gelida.Protein science : a publication of the Protein Society · 2025Article
- Enzymatic functionalization of bacterial nanocellulose: current approaches and future prospects.Journal of nanobiotechnology · 2025Review
- LPMO-Catalyzed Oxidation of Cellulosic Fibers with Controlled Addition of a Reductant and HACS sustainable chemistry & engineering · 2025Article
- A modular enzyme with combined hemicellulose-removing and LPMO activity increases cellulose accessibility in softwood.The FEBS journal · 2025Article
- Engineering the secretome of Aspergillus niger for cellooligosaccharides production from plant biomass.Microbial cell factories · 2024Article
- The impact of the carbohydrate-binding module on how a lytic polysaccharide monooxygenase modifies cellulose fibers.Biotechnology for biofuels and bioproducts · 2024Article
- Transcriptome-wide analysis of a superior xylan degrading isolate Penicillium oxalicum 5-18 revealed active lignocellulosic degrading genes.Archives of microbiology · 2024Article
- Enhancing enzymatic saccharification yields of cellulose at high solid loadings by combining different LPMO activities.Biotechnology for biofuels and bioproducts · 2024Article
- Discovery of a Novel Cellobiose Dehydrogenase fromJournal of microbiology and biotechnology · 2024Article
- TheApplied and environmental microbiology · 2023Article
- Resolving domain positions of cellobiose dehydrogenase by small angle X-ray scattering.The FEBS journal · 2023Article
- Efficient Secretory Production of Lytic Polysaccharide MonooxygenaseInternational journal of molecular sciences · 2023Article
- Revisiting the role of electron donors in lytic polysaccharide monooxygenase biochemistry.Essays in biochemistry · 2023Review
- A fungal lytic polysaccharide monooxygenase is required for cell wall integrity, thermotolerance, and virulence of the fungal human pathogen Cryptococcus neoformans.PLoS pathogens · 2023Article
- Lytic polysaccharide monooxygenases: enzymes for controlled and site-specific Fenton-like chemistry.Essays in biochemistry · 2023Article
- Biobased Nanomaterials─The Role of Interfacial Interactions for Advanced Materials.Chemical reviews · 2023Review
- Visible light-exposed lignin facilitates cellulose solubilization by lytic polysaccharide monooxygenases.Nature communications · 2023Article
45 more citing papers are in PubMed but not listed here.
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
Lytic polysaccharide monooxygenase (LPMO) represents a unique principle of oxidative degradation of recalcitrant insoluble polysaccharides. Used in combination with hydrolytic enzymes, LPMO appears to constitute a significant factor of the efficiency of enzymatic biomass depolymerization. LPMO activity on different cellulose substrates has been shown from the slow release of oxidized oligosaccharides into solution, but an immediate and direct demonstration of the enzyme action on the cellulose surface is lacking. Specificity of LPMO for degrading ordered crystalline and unordered amorphous cellulose material of the substrate surface is also unknown. We show by fluorescence dye adsorption analyzed with confocal laser scanning microscopy that a LPMO (from Neurospora crassa) introduces carboxyl groups primarily in surface-exposed crystalline areas of the cellulosic substrate. Using time-resolved in situ atomic force microscopy we further demonstrate that cellulose nano-fibrils exposed on the surface are degraded into shorter and thinner insoluble fragments. Also using atomic force microscopy, we show that prior action of LPMO enables cellulases to attack otherwise highly resistant crystalline substrate areas and that it promotes an overall faster and more complete surface degradation. Overall, this study reveals key characteristics of LPMO action on the cellulose surface and suggests the effects of substrate morphology on the synergy between LPMO and hydrolytic enzymes in cellulose depolymerization.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.